Results 121 to 130 of about 4,171,981 (305)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

open access: yesFEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti   +5 more
wiley   +1 more source

Flow loop plugging experiments: experimental logs

open access: yes
Flow loop sensor logs for plugging flow experiments which are described in the associated articles. The logs present experimental pressure, temperature, and mass flow rate measurements conducted at different places of the multiphase flow loop loaded with
Øye, Vegar H.   +2 more
core   +1 more source

Closed loop identification based on quantization [PDF]

open access: yes, 2002
This paper proposes a new closed-loop identification scheme for a single-input-single-output control loop. It is based upon a quantizer inserted into the feedback path. The quantizer can be used to generate an equivalent persistently exciting signal with
Huang, B, Wang, M, Thornhill, NF
core  

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

open access: yesFEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta   +7 more
wiley   +1 more source

Demo 06-09 Loop the Loop

open access: yes, 2001
A ball is rolled down an incline, as shown in Figure 1, performing a loop the loop at the bottom. For the ball to remain in contact with the incline at all times it must start at a height that will give it sufficient velocity at the top of the loop ...

core  

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

open access: yesFEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey   +4 more
wiley   +1 more source

A Loop Splitting Method of Irregular and Flow Dependence Loops

open access: yesIndian Journal of Science and Technology, 2015
A lot of work has been done in parallelizing nested loops with uniform dependences, from dependence analysis to loop transformation. Loops with non-uniform dependences are not so uncommon in the real world. This paper proposes an efficient method of splitting and transforming nested loops with irregular and flow dependences for maximizing parallelism ...
openaire   +1 more source

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

open access: yesFEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

Elimination of false-locking in long loop phase-locked receivers [PDF]

open access: yes, 1982
Although long-loop phase-locked techniques are finding increasing application in the fields of mobile radio and satellite communications, their use has several drawbacks.
Sladen, JPH, McGeehan, JP
core  

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