Results 151 to 160 of about 5,359,566 (332)
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
On fluid approximation for stable networks
Résumé disponible dans le fichier ...
Botvich, Dmitri D., Zamyatin, A.A.
openaire +1 more source
Approximation Error Bounds via Rademacher's Complexity [PDF]
Approximation properties of some connectionistic models, commonly used to construct approximation schemes for optimization problems with multivariable functions as admissible solutions, are investigated.
Gnecco, Giorgio, Sanguineti, Marcello
core
Cosmological models constructed by van der Waals fluid approximation and volumetric expansion
The universe modeled with van der Waals fluid approximation, where the van der Waals fluid equation of state contains a single parameter [Formula: see text].
G. C. Samanta, R. Myrzakulov
core +1 more source
Numerical Solution of an Inverse Problem of Filtration [PDF]
A numerical method is suggested to solve the multidimensional inverse problem for the fluid flow in a porous media in order to determine the flow rates by the given bottomhole pressure.
P.N. Vabishchevich +3 more
doaj
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Geometric fluid approximation for general continuous-time Markov chains. [PDF]
Michaelides M +2 more
europepmc +1 more source
The diffuse interface approximation to fluid-structure interaction
We consider a fluid-structure interaction problem in the Eulerian, phase-field formulation. The problem is described using the Navier--Stokes equations for a viscous, incompressible fluid, coupled with the incompressible hyperelasticity system, both written in the Eulerian coordinates.
Francis R. A. Aznaran +2 more
openaire +2 more sources

