Results 131 to 140 of about 1,778,184 (292)
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
FEBS Open Bio, EarlyView.Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
On fluid approximation for stable networks
, 1994 Résumé disponible dans le fichier ...
Botvich, Dmitri D., Zamyatin, A.A.
openaire +1 more source
Numerical Solution of an Inverse Problem of Filtration [PDF]
Учёные записки Казанского университета: Серия Физико-математические науки, 2015 A numerical method is suggested to solve the multidimensional inverse problem for the fluid flow in a porous media in order to determine the flow rates by the given bottomhole pressure.
P.N. Vabishchevich +3 more
doaj
Adenosine triphosphate as a modulator of protein interactions and stability
FEBS Open Bio, EarlyView.ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
Investigations of smoothed particle hydrodynamics method for fluid-rigid body interactions
, 2013 The aim of this project is to investigate the capability of smoothed particle hydrodynamics (SPH) method for fluid-rigid body interactions. SPH is one of the most widely used meshless methods which use particles to represent the system.
Sun, Fanfan
core +1 more source
Geometric fluid approximation for general continuous-time Markov chains. [PDF]
Proc Math Phys Eng Sci, 2019 Michaelides M +2 more
europepmc +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Variational inequations and approximation [PDF]
, 1974 The existence and uniqueness of the solution of a variational inequality is considered, and methods of approximation of the solution are given.
Noor, MA
core +1 more source