Results 51 to 60 of about 111,384 (304)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

open access: yesFEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho   +3 more
wiley   +1 more source

Influence of Fly Ash on the Fluidity of Blast Furnace Slag for the Preparation of Slag Wool

open access: yesCrystals, 2023
Using fly ash as the modifier, blast furnace slag was modified to prepare slag wool, fulfilling the goal of using one type of waste to make use of another type of waste, and it is of great significance for the comprehensive utilization of industrial bulk
Peipei Du   +3 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

open access: yesFEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes   +4 more
wiley   +1 more source

Preparation and Performance Testing of Steel Slag Concrete from Steel Solid Waste

open access: yesBuildings
In this paper, steel slag powder was used to replace part of the cement in road concrete, and group tests were carried out on coarse aggregates with different water–cement (W/C) ratios, different steel slag parameters, and different particle sizes.
Bao Yuan   +3 more
doaj   +1 more source

Numerical simulation and mathematical modeling of the casting process for pearlitic spheroidal graphite cast iron

open access: yesOpen Chemistry, 2022
Fluidity, an essential parameter in casting operations, is influenced by the thermal properties of the alloy and the mold, pouring temperature, modification, and inoculation of the alloy.
Şensoy Abdullah Tahir
doaj   +1 more source

Effects of kidney perfusion on renal stiffness and tissue fluidity measured with tomoelastography in an MRI-compatible ex vivo model

open access: yesFrontiers in Bioengineering and Biotechnology, 2023
Stiffness plays a vital role in diagnosing renal fibrosis. However, perfusion influences renal stiffness in various chronic kidney diseases. Therefore, we aimed to characterize the effect of tissue perfusion on renal stiffness and tissue fluidity ...
Johannes Castelein   +8 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

open access: yesFEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto   +3 more
wiley   +1 more source

Assessment of membrane fluidity in individual yeast cells by Laurdan generalised polarisation and multi-photon scanning fluorescence microscopy [PDF]

open access: yes, 2002
Here we describe techniques that we developed for monitoring membrane fluidity of individual yeast cells during environmental adaptation and physiological changes.
Learmonth, Robert P., Gratton, Enrico
core  

Membrane Fluidity in Yeast Adaptation: Insights from Fluorescence Spectroscopy and Microscopy

open access: yes, 2011
This review summarises work determining relationships among yeast membrane fluidity, cell physiology and environmental changes, utilising fluorescence spectroscopy and multi-photon microscopy.
Learmonth, Robert P.   +1 more
core   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

open access: yesFEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy   +4 more
wiley   +1 more source

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