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Proteolysis of fluoresceinated proteins
Molecular Immunology, 1965Abstract Pronase digestion of fluoresceinated BSA, fluroresceinated human and rabbit γ-globulin, and fluoresceinated BSA-rabbit anti-BSA precipitate was studied both as a function of time, and when digestion was complete, by separation of the products by gel fibration on Sephadex G-100.
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Time-resolved fluorescence properties of fluorescein and fluorescein glucuronide
Experimental Eye Research, 1989The use of fluorescein as a tracer in the study of the blood-ocular barriers is complicated by the metabolic production of fluorescein monoglucuronide, the excitation and fluorescence spectra of which overlap with those of fluorescein. Time-resolved fluorescence measurements provide a means of detecting the two substances in a mixture.
Michael Larsen, Lennart B.-Å. Johansson
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Studies on fluorescein—VIIThe fluorescence of fluorescein as a function of pH
Talanta, 1989The relative fluorescence of fluorescein over the pH range 3-12 has been measured at 516 nm, with excitation at 489 nm. The relative fluorescence is essentially zero at pH 3, increases slowly between pH 4 and 5, rises rapidly between pH 6 and 7, reaches a maximum at pH 8, and remains constant at above pH 8.
Richard Markuszewski, Harvey Diehl
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Fluorescein-guided surgery for malignant gliomas: a review
Neurosurgical Review, 2014Francesco Acerbi +2 more
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Ocular fluorescein phototoxicity
Applied Optics, 1987Fluorescein angiography is one of the most commonly used diagnostic techniques in modern ophthalmology. Prior to this study, recommendations on the phototoxicity of this technique were based on light levels alone (i.e., without fluorescein present). Using a rabbit model to demonstrate retinal damage, intravenous sodium fluorescein was found to reduce ...
Bernard F. Hochheimer +2 more
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Intravenous fluorescein overdose in a child undergoing fluorescein angiography
Journal of American Association for Pediatric Ophthalmology and StrabismusFluorescein angiography is a fluorescent dye-based imaging procedure, most commonly indicated in the pediatric setting to evaluate peripheral retinal vascular lesions. Fluorescein dye is organic, water soluble, and largely excreted renally, with a reassuring safety profile at therapeutic doses. While toxicity with intrathecal overdose has been reported,
Rhea W. Teng +3 more
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Observations on the absorption spectra of fluorescein, fluorescein derivatives and conjugates
Archives of Biochemistry and Biophysics, 1958Abstract 1. 1. Rapid loss in the characteristic maximum at 490 mμ occurs in solutions of fluorescein (uranin) below pH 7.0 with elimination of fluorescence at pH 2.0. A new absorption band appears in these solutions below pH 5.0 with a maximum at 430 mμ at pH 2.0. 2. 2.
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Endoscopic Fluorescein Angiography
Ophthalmic Surgery, Lasers and Imaging Retina, 1996* BACKGROUND AND OBJECTIVE: To assess features of various retinal and choroidal disorders by endoscopic fluorescein angiograms (EFAs) obtained intraoperatively. * PATIENTS AND METHODS: One hundred patients undergoing vitrectomy were studied intraoperatively by injecting 5 ml of 10% fluorescein intravenously.
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The Use of Fluorescein in Vitrectomy
Archives of Ophthalmology, 1987To the Editor. —Fluorescein has been proposed as an aid in vitreous surgery. This agent can be injected intravenously prior to operation 1 or introduced directly into the eye. 2 Intravenous injection carries the risk of serious systemic reaction and is also of little use in unplanned procedures, since the dye has to be injected at least 18 hours ...
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