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The Photography of Fluorescein

Plastic and Reconstructive Surgery, 1982
The last few years have seen a number of new flaps described and a renewed interest in the use of fluorescein, but there have been few photographs of the fluorescein effect, because special light sources were required with the filters that were employed.
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Fluorescein Angiography

2018
Fluorescein angiography (FA) is an imaging modality used to study the circulation of the retina and choroid. About 5 mL of 10% sterile aqueous solution of fluorescein dye (or 2-3 mL of a 25% solution) is injected in the antecubital vein. The fluorescein molecules excited by a blue light (465-490 nm) emit green-yellow light (520-530 nm) (Fig. 2.1).
Stephen H, Tsang, Tarun, Sharma
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Fluorescein by XPS

Surface Science Spectra, 1992
X-ray photoemission spectra of the core and valence levels for fluorescein are presented. The data were collected on a Perkin-Elmer/Physical Electronics model #5400 photoelectron spectrometer using unmonochromatized Mg x rays. The material, in powder form, was analyzed in the as received condition. Fluorescein is an important prototype organic dye with
Bruce C. Beard   +3 more
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Mechanism of quenching of fluorescein by anti-fluorescein IgG antibodies

Immunochemistry, 1977
Abstract The mechanism by which fluorescein underoges a spectral shift and its fluorescence is quenched when bound to rabbit anti-flurescyl IgG antibody, has been investigated. Visible absorption spectra of the fluorophore bound to the active site of purified antibody elicited to the two isomers of fluorescein (I and II) revealed different spectral ...
Edward W. Voss, Robert M. Watt
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Studies on fluorescein—VIIThe fluorescence of fluorescein as a function of pH

Talanta, 1989
The relative fluorescence of fluorescein over the pH range 3-12 has been measured at 516 nm, with excitation at 489 nm. The relative fluorescence is essentially zero at pH 3, increases slowly between pH 4 and 5, rises rapidly between pH 6 and 7, reaches a maximum at pH 8, and remains constant at above pH 8.
Richard Markuszewski, Harvey Diehl
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Successful fluorescein desensitization

Allergy, 2003
FLUORESCEIN ...
Nucera, Eleonora   +8 more
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Proteolysis of fluoresceinated proteins

Molecular Immunology, 1965
Abstract Pronase digestion of fluoresceinated BSA, fluroresceinated human and rabbit γ-globulin, and fluoresceinated BSA-rabbit anti-BSA precipitate was studied both as a function of time, and when digestion was complete, by separation of the products by gel fibration on Sephadex G-100.
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Free Plasma Fluorescein

Archives of Ophthalmology, 1985
To the Editor. —In our experiments with vitreous fluorophotometry, we measured levels of free plasma sodium fluorescein using a pressure dialysis technique (Amicon ultrafiltration membranes). We have made someobservations with this technique that other investigators may find helpful.
Andrew K. Vine, Anne Kisly
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Time-resolved fluorescence properties of fluorescein and fluorescein glucuronide

Experimental Eye Research, 1989
The use of fluorescein as a tracer in the study of the blood-ocular barriers is complicated by the metabolic production of fluorescein monoglucuronide, the excitation and fluorescence spectra of which overlap with those of fluorescein. Time-resolved fluorescence measurements provide a means of detecting the two substances in a mixture.
Michael Larsen, Lennart B.-Å. Johansson
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Intravenous fluorescein overdose in a child undergoing fluorescein angiography

Journal of American Association for Pediatric Ophthalmology and Strabismus
Fluorescein angiography is a fluorescent dye-based imaging procedure, most commonly indicated in the pediatric setting to evaluate peripheral retinal vascular lesions. Fluorescein dye is organic, water soluble, and largely excreted renally, with a reassuring safety profile at therapeutic doses. While toxicity with intrathecal overdose has been reported,
Rhea W. Teng   +3 more
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