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Fluorescent Immunohistochemistry
2021Immunofluorescence is an important immunochemical technique that utilizes fluorescence-labeled antibodies to detect specific target antigens. It is used widely in both scientific research and clinical laboratories. Immunofluorescence allows for excellent sensitivity and amplification of signal in comparison to immunohistochemistry. However, analysis of
Vanessa, Moreno +2 more
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2003
This chapter has introduced the theory of fluorescence, and briefly shown that it is a technique with many wide and varied applications in biochemistry. It has the overall advantage of being able to carry out measurements on small amounts of sample in solution, often revealing important information concerning the structure and dynamics of these systems.
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This chapter has introduced the theory of fluorescence, and briefly shown that it is a technique with many wide and varied applications in biochemistry. It has the overall advantage of being able to carry out measurements on small amounts of sample in solution, often revealing important information concerning the structure and dynamics of these systems.
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2019
In this two-part chapter, the background to confocal microscopy and two-photon fluorescence microscopy is first presented, with a detailed description of the optical setup. This is followed by a critical account of the many super-resolution techniques: coordinated stochastic fluorescence microscopy (photoactivation localization microscopy ...
Alberto Diaspro +7 more
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In this two-part chapter, the background to confocal microscopy and two-photon fluorescence microscopy is first presented, with a detailed description of the optical setup. This is followed by a critical account of the many super-resolution techniques: coordinated stochastic fluorescence microscopy (photoactivation localization microscopy ...
Alberto Diaspro +7 more
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Clinics in Plastic Surgery, 2011
Fluorescent angiography is a simple and effective real-time tool for measurement of tissue perfusion both in and out of the operating room. It has multiple uses including: (1) identifying perforating vessels during flap planning; (2) locating primary and secondary angiosomes within a prepared flap; (3) as an aid in decision making for tissue ...
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Fluorescent angiography is a simple and effective real-time tool for measurement of tissue perfusion both in and out of the operating room. It has multiple uses including: (1) identifying perforating vessels during flap planning; (2) locating primary and secondary angiosomes within a prepared flap; (3) as an aid in decision making for tissue ...
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Nature Methods, 2005
Although fluorescence microscopy permeates all of cell and molecular biology, most biologists have little experience with the underlying photophysical phenomena. Understanding the principles underlying fluorescence microscopy is useful when attempting to solve imaging problems.
Jeff W, Lichtman, José-Angel, Conchello
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Although fluorescence microscopy permeates all of cell and molecular biology, most biologists have little experience with the underlying photophysical phenomena. Understanding the principles underlying fluorescence microscopy is useful when attempting to solve imaging problems.
Jeff W, Lichtman, José-Angel, Conchello
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2013
Optical microscopy has developed as an indispensable tool for Arabidopsis cell biology. This is due to the high sensitivity, good spatial resolution, minimal invasiveness, and availability of autofluorescent proteins, which can be specifically fused to a distinct protein of interest.
Sébastien, Peter +2 more
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Optical microscopy has developed as an indispensable tool for Arabidopsis cell biology. This is due to the high sensitivity, good spatial resolution, minimal invasiveness, and availability of autofluorescent proteins, which can be specifically fused to a distinct protein of interest.
Sébastien, Peter +2 more
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Human Pathology, 1984
Fluorescence immunoassay is a sensitive technique that can be used in the measurement of many compounds, including drugs, hormones, and proteins; in the identification of antibodies; and in the quantification of antigens such as viral particles and, potentially, bacteria. Homogeneous fluorescence immunoassay, fluorescent excitation transfer immunoassay,
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Fluorescence immunoassay is a sensitive technique that can be used in the measurement of many compounds, including drugs, hormones, and proteins; in the identification of antibodies; and in the quantification of antigens such as viral particles and, potentially, bacteria. Homogeneous fluorescence immunoassay, fluorescent excitation transfer immunoassay,
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FLUORESCENCE AND FLUORESCENCE CONVERSION
1965Abstract : The quenching constant for the iodide quenching of the fluorescence of riboflavin phosphate was measured at pH 5 and at pH 8. The value of this constant (K1) at the lower pH (where the phosphate group is unionized), was independent of the ionic strength, mu.
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