Results 121 to 130 of about 69,474 (333)

Injectable Dual‐Network Hydrogel System for Osteochondral Repair Combining Immunomodulation, Mechanical Adaptability, and Enhanced Tissue Integration

open access: yesAdvanced Functional Materials, EarlyView.
A UV‐triggered injectable dual‐network hydrogel is reported as the first application of bletilla striata polysaccharide (BSP) in osteochondral repair. By integrating methacrylamide‐modified BSP and nitrobenzaldehyde‐functionalized hyaluronic acid, the system achieves immunomodulation, mechanical reinforcement, and dynamic tissue adhesion, thereby ...
Jiaming Cui   +10 more
wiley   +1 more source

True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy

open access: yesNature Communications, 2018
Methods to measure selective transcription factor-DNA binding often lack sensitivity and are not performed in solution. Here the authors develop a method to perform fluorescence anisotropy measurements of transcription factor-DNA binding energies with ...
Christophe Jung   +6 more
doaj   +1 more source

Determination of Adeno-associated Virus Rep DNA Binding Using Fluorescence Anisotropy

open access: yesBio-Protocol, 2017
Quantitative measurement of proteins binding to DNA is a requisite to fully characterize the structural determinants of complex formation necessary to understand the DNA transactions that regulate cellular processes.
Francisco Zarate-Perez   +6 more
doaj   +1 more source

Time-resolved fluorescence anisotropy of fluorescent-labeled lysophospholipid and taurodeoxycholate aggregates

open access: yesBiophysical Journal, 1996
Previous work from this laboratory demonstrated that the environment-sensitive lysolipid N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)- monomyristoylphosphatidylethanolamine (N-NBD-MPE), at concentrations below its critical micelle concentration (CMCN-NBD-MPE = 4 microM), reached maximum fluorescence yield upon the addition of taurodeoxycholate (TDC) at ...
DeLong, L.J., Nichols, J.W.
openaire   +2 more sources

Micro and Nanostructural Diversity of Lizard Osteoderm Capping Tissue in Relation to Mechanical Performance

open access: yesAdvanced Functional Materials, EarlyView.
This study shows that lizard osteoderm capping tissue is a hyper‐mineralized hydroxyapatite layer consistently covering the superficial osteoderm surface in those species studied here, yet it varies greatly in morphology, nanostructure, and mechanical performance across species.
Adrian Rodriguez‐Palomo   +10 more
wiley   +1 more source

Mimicking Block Copolymer Self‐Assembly with One‐Pot Synthesized Polyphosphoester Gradient Copolymers

open access: yesAdvanced Functional Materials, EarlyView.
Degradable Polyphosphoester (PPE) gradient copolymers (GCPs) are synthesized via one‐pot copolymerization. We show that GCPs self‐assemble into nanostructures like polymersomes, effectively mimicking the behavior of traditional BCPs. The gradient strength is key, with softer gradients favoring micelles.
Suna Azhdari   +7 more
wiley   +1 more source

A fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin

open access: yesNature Communications, 2018
Microtubule-targeting agents are used successfully as anticancer therapeutics. Here authors develop a fluorescence-anisotropy-based assay to identify and characterize ligands for the maytansine site of tubulin and provide crystal structures of identified
Grégory Menchon   +10 more
doaj   +1 more source

Conductive Bonding and System Architectures for High‐Performance Flexible Electronics

open access: yesAdvanced Functional Materials, EarlyView.
This review outlines bonding technologies and structural design strategies that support high‐performance flexible and stretchable electronics. Bonding approaches such as surface‐activated bonding and anisotropic conductive films, together with system‐level architectures including buffer layers and island‐bridge structures, possess distinct mechanical ...
Kazuma Nakajima, Kenjiro Fukuda
wiley   +1 more source

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