Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein. [PDF]
Micromachines (Basel), 2021 Fluorescence anisotropy, a technique to study the folding state of proteins or affinity of ligands, is used in this present work as a temperature sensor, to measure the microfluidic temperature field, by adding fluorophore in the liquid.
Jain P, Aida T, Motosuke M.
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Pressure and temperature dependence of fluorescence anisotropy of green fluorescent protein. [PDF]
RSC Adv, 2022 We have studied the effect of high hydrostatic pressure and temperature on the steady state fluorescence anisotropy of Green Fluorescent Protein (GFP). We find that the fluorescence anisotropy of GFP at a constant temperature decreases with increasing pressure.
Kaur H, Nguyen K, Kumar P.
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DNA tetrahedron for fluorescence anisotropy of intercalators
He jishu, 2021 BackgroundThe fluorescence anisotropy of intercalative dye plays important roles in nucleic acid analysis, the construction of fluorescent probes, and biosensing.
HUANG Qiuling +4 more
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Live-cell microscopy or fluorescence anisotropy with budded baculoviruses-which way to go with measuring ligand binding to M4 muscarinic receptors? [PDF]
Open Biol, 2022 M4 muscarinic acetylcholine receptor is a G protein-coupled receptor (GPCR) that has been associated with alcohol and cocaine abuse, Alzheimer's disease, and schizophrenia which makes it an interesting drug target.
Tahk MJ +10 more
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Photonics, 2022 The fluorescence anisotropy of photosensitizers Radachlorin and chlorin e6 was studied using the time-resolved single photon-counting technique under one- and two-photon excitation within the Soret absorption band.
Ioanna A. Gorbunova +6 more
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Fluorescence Anisotropy Sensor Comprising a Dual Hollow-Core Antiresonant Fiber Polarization Beam Splitter. [PDF]
Sensors (Basel), 2020 Fluorescence anisotropy imaging and sensing is a widely recognized method for studying molecular orientation and mobility. However, introducing this technique to in vivo systems is a challenging task, especially when one considers multiphoton excitation ...
Stawska HI, Popenda MA.
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The Role of the Photon Counting Loss Effect in Time-Resolved Measurements of Fluorescence Anisotropy
Photonics, 2023 Determining the rate of rotation of molecules from their fluorescence anisotropy decay curves is a powerful method for studying molecular systems in biological applications.
Daniil A. Gvozdev +3 more
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Polarization modulation with optical lock-in detection reveals universal fluorescence anisotropy of subcellular structures in live cells. [PDF]
Light Sci Appl, 2022 The orientation of fluorophores can reveal crucial information about the structure and dynamics of their associated subcellular organelles. Despite significant progress in super-resolution, fluorescence polarization microscopy remains limited to unique ...
Guan M +13 more
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Triple Fluorescence Anisotropy Reporter Imaging in Living Cells. [PDF]
Bio Protoc, 2019 FRET-based genetically encoded biosensors incorporate two fluorescent proteins into their design to enable ratiometric biosensing of signaling activities in live cells.
Ross B +4 more
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Confocal fluorescence anisotropy and FRAP imaging of α-synuclein amyloid aggregates in living cells. [PDF]
PLoS ONE, 2011 We assessed the intracellular association states of the Parkinson's disease related protein α-synuclein (AS) in living cells by transfection with a functional recombinant mutant protein (AS-C4) bearing a tetracysteine tag binding the fluorogenic ...
M Julia Roberti +2 more
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