Results 31 to 40 of about 69,474 (333)

Reynolds-number effects and anisotropy in transverse-jet mixing [PDF]

open access: yes, 2006
Experiments are described which measured concentration fields in liquid-phase strong transverse jets over the Reynolds-number range 1.0×10^3 ≤ Rej ≤ 20×10^3. Laser-induced-fluorescence measurements were made of the jet-fluid-concentration fields at a jet-
Dimotakis, Paul E., Shan, Jerry W.
core   +1 more source

Methods toward simplification of time resolved fluorescence anisotropy in proteins labeled with NBD (4-chloro-7-nitrobenzofurazan) adducts

open access: yesMethodsX, 2019
The analysis of time resolved fluorescence anisotropy for NBD tagged proteins is difficult when multiple exponential components arise from heterogeneous amino acid fluorescent adducts. Two approaches were taken toward simplification.
Ben J. Glasgow, Adil R. Abduragimov
doaj   +1 more source

Super-resolution fluorescence polarization microscopy [PDF]

open access: yesJournal of Innovative Optical Health Sciences, 2018
Fluorescence polarization is related to the dipole orientation of chromophores, making fluorescence polarization microscopy possible to reveal structures and functions of tagged cellular organelles and biological macromolecules.
Karl Zhanghao   +4 more
doaj   +1 more source

Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]

open access: yes, 2012
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Abbe   +295 more
core   +2 more sources

Interfacial Recognition of Acetylcholine by an Amphiphilic p-Sulfonatocalix[8]arene Derivative Incorporated into Dimyristoyl Phosphatidylcholine Vesicles

open access: yesSensors, 2008
Dodecyl ether derivatives 1-3 of p-sulfonatocalix[n]arene were incorporated into dimyristoyl phosphatidylcholine (DMPC) vesicles, and their binding abilities for acetylcholine (ACh) were examined by using steady-state fluorescence/fluorescence anisotropy
Yasuhiro Ooi   +2 more
doaj   +1 more source

Sensing ligand binding to a clinically relevant lectin by tryptophan fluorescence anisotropy [PDF]

open access: yes, 2011
Increasing insights into the involvement of endogenous lectins in disease processes fuel the interest to develop potent inhibitors. As a consequence, robust assay procedures are required. Due to their activity as adhesion/growth-regulatory effectors this
André, Sabine   +5 more
core   +1 more source

Anionic Phospholipids Shift the Conformational Equilibrium of the Selectivity Filter in the KcsA Channel to the Conductive Conformation: Predicted Consequences on Inactivation

open access: yesBiomedicines, 2023
Here, we report an allosteric effect of an anionic phospholipid on a model K+ channel, KcsA. The anionic lipid in mixed detergent–lipid micelles specifically induces a change in the conformational equilibrium of the channel selectivity filter (SF) only ...
María Lourdes Renart   +4 more
doaj   +1 more source

Investigations of excitation energy transfer and intramolecular interactions in a nitrogen corded distrylbenzene dendrimer system. [PDF]

open access: yes, 2002
The photophysics of an amino-styrylbenzene dendrimer (A-DSB) system is probed by time-resolved and steady state luminescence spectroscopy. For two different generations of this dendrimer, steady state absorption, emission, and photoluminescence ...
Becky Letchford   +15 more
core   +4 more sources

Steady-state acceptor fluorescence anisotropy imaging under evanescent excitation for visualisation of FRET at the plasma membrane.

open access: yesPLoS ONE, 2014
We present a novel imaging system combining total internal reflection fluorescence (TIRF) microscopy with measurement of steady-state acceptor fluorescence anisotropy in order to perform live cell Förster Resonance Energy Transfer (FRET) imaging at the ...
Viviane Devauges   +10 more
doaj   +1 more source

Loss of plasma membrane lipid asymmetry can induce ordered domain (raft) formation

open access: yesJournal of Lipid Research, 2022
In some cases, lipids in one leaflet of an asymmetric artificial lipid vesicle suppress the formation of ordered lipid domains (rafts) in the opposing leaflet. Whether this occurs in natural membranes is unknown.
Shinako Kakuda   +3 more
doaj   +1 more source

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