Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association. [PDF]
Nat Commun, 2020 Performing homo-FRET measurements in cells using a fluorescence microscope is challenging, especially when using high numerical aperture objective lenses.
Ojha N, Rainey KH, Patterson GH.
europepmc +2 more sources
Molecules, 2022 The influence of chitosan (CS) and amphiphilic polymers (AP: pluronic F108 and polyvinylpyrrolidone (PVP)) on the photocatalytic activity of rose bengal (RB) in a model reaction of tryptophan photo-oxidation in phosphate-buffered saline (PBS) was studied.
Anastasia S. Kuryanova +8 more
doaj +1 more source
Atomistic picture of fluorescent probes with hydrocarbon tails in lipid bilayer membranes: an investigation of selective affinities and fluorescent anisotropies in different environmental phases [PDF]
Langmuir 2018, 34, 9072-9084, 2019 By reverting to spectroscopy, changes in the biological environment of a fluorescent probe can be monitored and the presence of various phases of the surrounding lipid bilayer membranes can be detected. This study highlights the important differences in orientation and location and therefore in efficiency between the probes when they are used in ...
arxiv +1 more source
Conformational Transitions Accompanying Oligomerization of Yeast Alcohol Oxidase, a Peroxisomal Flavoenzyme [PDF]
, 1999 Alcohol oxidase (AO) is a homo-octameric flavoenzyme which catalyzes methanol oxidation in methylotrophic yeasts. AO protein is synthesized in the cytosol and subsequently sorted to peroxisomes where the active enzyme is formed.
Boteva, R +6 more
core +3 more sources
Sensors, 2008 Dodecyl ether derivatives 1-3 of p-sulfonatocalix[n]arene were incorporated into dimyristoyl phosphatidylcholine (DMPC) vesicles, and their binding abilities for acetylcholine (ACh) were examined by using steady-state fluorescence/fluorescence anisotropy
Yasuhiro Ooi +2 more
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Influence of Membrane Phase on the Optical Properties of DPH
Molecules, 2020 The fluorescent molecule diphenylhexatriene (DPH) has been often used in combination with fluorescence anisotropy measurements, yet little is known regarding the non-linear optical properties.
Silvio Osella +3 more
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Chemosensors, 2022 Biomacromolecules have been employed successfully as fluorescence anisotropy (FA) amplifiers for biosensing in reported studies. However, the sensitivities of the traditional biomacromolecule amplified FA strategies need to be improved because of the ...
Tianjin Xie +7 more
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Non-linear optical spectroscopy and two-photon excited fluorescence spectroscopy reveal the excited states of fluorophores embedded in beetle's elytra [PDF]
Interface Focus 9 (2018) 20180052, 2018 Upon illumination by ultraviolet light, many animal species emit light through fluorescence processes arising from fluorophores embedded within their biological tissues. Fluorescence studies in living organisms are however relatively scarce and so far limited to the linear regime.
arxiv +1 more source
Super-resolution fluorescence polarization microscopy [PDF]
Journal of Innovative Optical Health Sciences, 2018 Fluorescence polarization is related to the dipole orientation of chromophores, making fluorescence polarization microscopy possible to reveal structures and functions of tagged cellular organelles and biological macromolecules.
Karl Zhanghao +4 more
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WawHelioGlow: a model of the heliospheric backscatter glow. II. The helioglow buildup and the potential significance of the anisotropy in the solar EUV output [PDF]
, 2021 The helioglow is a fluorescence of interstellar atoms inside the heliosphere, where they are excited by the solar EUV. Because the mean free path between collisions for the interstellar gas is comparable to the size of the heliosphere, the distribution function of this gas inside the heliosphere strongly varies in space and with time and is non ...
arxiv +1 more source