Results 41 to 50 of about 69,474 (333)
Mechanism of DNA Recognition at a Viral Replication Origin [PDF]
, 2006 Recognition of the DNA origin by the Epstein-Barr nuclear antigen 1 (EBNA1) protein is the primary event in latentphase genome replication of the Epstein-Barr virus, a model for replication initiation in eukaryotes.
de Prat Gay, Gonzalo +3 more
core +1 more source
Polar Fluorenes and Spirobifluorenes: Fluorescence and Fluorescence Anisotropy Spectra
The Journal of Physical Chemistry B, 2011 An extensive spectroscopic study is presented of two asymmetrically substituted fluorene dyes and of the related spiro dimers in both liquid and glassy solvents. Essential-state models are developed to accurately reproduce linear absorption and fluorescence spectra and their complex dependence on solvent polarity.
Grisanti L +6 more
openaire +7 more sources
A multi-functional imaging approach to high-content protein interaction screening.
PLoS ONE, 2012 Functional imaging can provide a level of quantification that is not possible in what might be termed traditional high-content screening. This is due to the fact that the current state-of-the-art high-content screening systems take the approach of ...
Daniel R Matthews +10 more
doaj +1 more source
Fluorescence anisotropy analysis of protein–antibody interaction [PDF]
Dyes and Pigments, 2009 Abstract The interaction between glutathione S -transferase and its antibody α-glutathione S -transferase (B-14) was studied using fluorescence anisotropy, subsequent to glutathione S -transferase bioconjugation with fluorescein-5-maleimide, leading to the determination of the dissociation and association binding constants, K d and K a ; good
BARBERO, Nadia +7 more
openaire +1 more source
Ras hyperactivation versus overexpression : Lessons from Ras dynamics in Candida albicans [PDF]
, 2018 We thank Prof. Neta Dean for the CIp10ADH1-Cherry plasmid and Prof. Aaron Mitchell for the BWP17 strain. We gratefully acknowledge Prof. Sudipta Maiti, TIFR, Mumbai, India for providing the data acquisition software.
Haokip, Dominic T +10 more
core +3 more sources
Properties of the mechanosensitive channel MscS pore revealed by tryptophan scanning mutagenesis [PDF]
, 2015 Funding This work was supported by a Wellcome Trust Programme grant [092552/A/10/Z awarded to I.R.B., S.M., J. H. Naismith (University of St Andrews, St Andrews, U.K.), and S. J. Conway (University of Oxford, Oxford, U.K.)] (T.R.
Akiko Rasmussen +12 more
core +4 more sources
Detection of Ras nanoclustering-dependent homo-FRET using fluorescence anisotropy measurements
European Journal of Cell Biology, 2023 The small GTPase Ras is frequently mutated in cancer and a driver of tumorigenesis. The recent years have shown great progress in drug-targeting Ras and understanding how it operates on the plasma membrane.
Ganesh babu Manoharan +3 more
doaj +1 more source
Influence of M-phase chromatin on the anisotropy of microtubule asters [PDF]
, 1996 In many eukaryotic cells going through M-phase, a bipolar spindle is formed by microtubules nucleated from centrosomes. These microtubules, in addition to being "captured" by kinetochores, may be stabilized by chromatin in two different ways: short-range
Dogterom, M. +3 more
core +2 more sources
Frontiers in Molecular Biosciences, 2017 PDZ domains generally bind short amino acid sequences at the C-terminus of target proteins, and short peptides can be used as inhibitors or model ligands.
Nicolas Panel +4 more
doaj +1 more source
BBA Advances, 2023 Emission fluorescence is one of the most versatile and powerful biophysical techniques used in several scientific subjects. It is extensively applied in the studies of proteins, their conformations, and intermolecular contacts, such as in protein-ligand ...
Fábio Henrique dos Santos Rodrigues +3 more
doaj +1 more source