Results 201 to 210 of about 74,021 (249)

Recent advances in fluorescence correlation spectroscopy

Current Opinion in Structural Biology, 2002
Fluorescence correlation spectroscopy is a method in which fluctuations in the fluorescence arising from a very small sample volume are correlated to obtain information about the processes giving rise to the fluctuations. Recent progress has been made in methodologies such as two-photon excitation, photon counting histogram analysis, cross-correlation,
Nancy L Thompson
exaly   +3 more sources

Fluorescence Lifetime Correlation Spectroscopy

Journal of Fluorescence, 2006
This article explains the basic principles of FLCS, a genuine fusion of Time-Correlated Single Photon Counting (TCSPC) and Fluorescence Correlation Spectroscopy (FCS), using common terms and minimum mathematics. The usefulness of the method is demonstrated on simple FCS experiments.
Peter, Kapusta, Michael, Wahl, Ales, Benda, Martin, Hof, Jörg, Enderlein   +4 more
openaire   +2 more sources

Fluorescence Correlation Spectroscopy

2007
Fluorescence correlation spectroscopy (FCS) is a technique that allows for an extremely sensitive determination of molecular diffusion properties, down to the level of single molecules. It thus provides an attractive alternative to FRAP, requiring much less laser power and lower concentrations of fluorophores.
Kirsten, Bacia, Petra, Schwille
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Noise on Fluorescence Correlation Spectroscopy

Journal of Colloid and Interface Science, 2001
The time dependence of the noise and the signal-to-noise (SN) ratio of the fluorescence correlation spectroscopy (FCS) autocorrelation function is obtained from replica measurements of standard dextran solutions. The noise dependence on the delay time is fitted by a hyperbolic function with two fitting parameters.
Starchev K, Ricka J, Buffle J
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Inverse-Fluorescence Correlation Spectroscopy

Analytical Chemistry, 2009
An alternative version of fluorescence correlation spectroscopy is presented, where the signal from a medium surrounding the particles of interest is analyzed, as opposed to a signal from the particles themselves. This allows for analysis of unlabeled particles and potentially of biomolecules.
Stefan, Wennmalm, Per, Thyberg, Lei, Xu, Jerker, Widengren   +3 more
openaire   +2 more sources

Microrheology with Fluorescence Correlation Spectroscopy

Langmuir, 2009
We show that fluorescence correlation spectroscopy (FCS) using a commercial spectrometer can be applied to passive microrheological (MR) experiments. The method probes the local rheological properties of materials on length scales of the focus dimension of the confocal microscope.
Rathgeber, S., Beauvisage, H-J., Chevreau, H., Willenbacher, N., Oelschlaeger, C.   +4 more
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Applications of Fluorescence Correlation Spectroscopy

Quarterly Reviews of Biophysics, 1976
The preceding paper by Douglas Magde has recounted the basic principles of Fluorescence Correlation Spectroscopy (FCS) as originally described (see Magde, Elson & Webb, 1972; Elson & Magde, 1974; Magde, Elson & Webb, 1974 Elson & Webb, 1975; referred to collectively as MEW), and has described the first application to chemical kinetics ...
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On the statistics of fluorescence correlation spectroscopy

Biophysical Chemistry, 1990
I present a detailed statistical analysis of fluorescence correlation spectroscopy (FCS) which is a natural extension of an early work. This analysis more realistically takes account of the following issues. (1) A spatial Gaussian laser excitation of fluorescence, (2) the effect of a small number of fluorescent molecules in the observation volume, (3 ...
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Fluorescence Correlation Spectroscopy

2014
Fluorescence fluctuation spectroscopy techniques allow the quantification of fluorescent molecules present at the nanomolar concentration level. After a brief introduction to the technique, this chapter presents a protocol including background information in order to measure and quantify the molecular interaction of two signaling proteins inside the ...
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Inverse-Fluorescence Cross-Correlation Spectroscopy

Analytical Chemistry, 2010
Inverse-fluorescence correlation spectroscopy (iFCS) was recently introduced as an alternative version of FCS that does not require labeling of the analyzed particles or biomolecules. In iFCS, the signal from a medium surrounding the particles is analyzed, as opposed to a signal from the studied particles themselves.
Stefan, Wennmalm, Jerker, Widengren
openaire   +2 more sources