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Fluorescence lifetime-resolved imaging
Photosynthesis Research, 2009This is a short account of fluorescence lifetime-resolved imaging, in order to acquaint readers who are not experts with the basic methods for measuring lifetime-resolved signals throughout an image. We present the early FLI (fluorescence lifetime imaging) history, review shortly the instrumentation and experimental design, discuss briefly the ...
Yi-Chun, Chen, Robert M, Clegg
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Fluorescence image-guided neurosurgery
Future Oncology, 2017Surgery plays an important role in the management of high-grade gliomas (HGG) and imparts significant tumor-free and overall survival advantages. However HGG margins are often invisible, making their gross total resection (GTR) a difficult task. Hence intraoperative technology such as intraoperative fluorescence was a revolutionary discovery.
Sadao, Kaneko, Muftah S, Eljamel
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Live‐Cell Fluorescence Imaging
2007This chapter examines the ways to optimize the signal-to-noise ratio while keeping the specimen healthy. Live cells expressing fluorescent protein fusions are usually dim compared to fixed specimens, both because the fluorescent proteins are not very bright and because there is, in most cases, only one fluorophores per protein.
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Spectral imaging fluorescence microscopy
Genes to Cells, 2002The spectral resolution of fluorescence microscope images in living cells is achieved by using a confocal laser scanning microscope equipped with grating optics. This capability of temporal and spectral resolution is especially useful for detecting spectral changes of a fluorescent dye; for example, those associated with fluorescence resonance energy ...
Tokuko, Haraguchi +4 more
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Fluorescence Lifetime Imaging Microscopy
2007Publisher Summary Fluorescence lifetime imaging microscopy (FLIM) produces spatially resolved images of fluorophore lifetime (the property describing how rapidly fluorescence decays), providing another dimension of information for visualizing fluorophores and an additional source of contrast.
Ching-Wei, Chang +2 more
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Fluorescence lifetime imaging of coral fluorescent proteins
Microscopy Research and Technique, 2007AbstractCorals, like many other coelenterates, contain fluorescent pigments that show considerable homology with the well known green fluorescent protein of the jellyfish Aequoria. In corals, unlike jellyfish, multiple proteins are present and the range of excitations and emissions suggest the possibility of energy transfer.
Guy, Cox, Mikhail, Matz, Anya, Salih
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2000
A number of fluorescence imaging techniques show diagnostic promise. Imaging endogenous fluorescence has been proposed as a method for cancer diagnosis. Unfortunately, tissue autofluorescence is relatively weak and poor contrast between malignant and normal tissue is seen.
Ramjiawan, Bram +2 more
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A number of fluorescence imaging techniques show diagnostic promise. Imaging endogenous fluorescence has been proposed as a method for cancer diagnosis. Unfortunately, tissue autofluorescence is relatively weak and poor contrast between malignant and normal tissue is seen.
Ramjiawan, Bram +2 more
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Confocal Fluorescence Lifetime Imaging
1996The optical microscope, arguably the most mature of all laboratory instruments, is currently enjoying a period of intense development that is opening up many new application areas. These developments have been made possible by the use of new optical arrangements, sophisticated light sources and detectors and by the application of novel fluorescence ...
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Molecular imaging in oncology: Current impact and future directions
Ca-A Cancer Journal for Clinicians, 2022Steven P Rowe, Martin G Pomper
exaly