Results 171 to 180 of about 79,470 (290)

Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding. [PDF]

Adv Drug Deliv Rev, 2023
Barroso M, Monaghan MG, Niesner R, Dmitriev RI.   +3 more
europepmc   +1 more source

A Guide for Spatial Omics Technologies: Innovation, Evaluation, and Application

Advanced Science, EarlyView.
This review presents a strategy‐centric framework for spatial omics technologies, organizing methods by how spatial information is experimentally encoded. It compares key performance trade‐offs across sequencing‐ and imaging‐based approaches, examines computational and practical limitations, and highlights biomedical applications. The analysis provides
Xiaofeng Wu, Weize Xu, Da Lin, Leqiang Sun, Jinxia Dai, Gang Cao   +5 more
wiley   +1 more source

Two-photon fluorescence lifetime imaging microscopy of NADH metabolism in HIV-1 infected cells and tissues. [PDF]

Front Immunol, 2023
Snyder GA, Kumar S, Lewis GK, Ray K.
europepmc   +1 more source

Endoplasmic Reticulum Geometry Dictates Neuronal Bursting via Calcium Store Refill Rates and Exposes Selective Neuronal Vulnerability

Advanced Science, EarlyView.
The ER's continuous tubular network is maintained by ER‐shaping proteins whose mutation or dysregulation contributes to neurodegenerative diseases. Here, we show that ER morphology sets the speed of Ca2+ store replenishment between firing events. Disrupting ER continuity slows intra‐ER Ca2+ redistribution from extracellular refill (SOCE) sites, driving
Valentina Davi, Pierre Parutto, Yuyi Zhang, Tasuku Konno, Cecile Crapart, Raquel Pereira, John P. Franklin, Mosab Ali Awadelkareem, Daniel C Maddison, Michael J. Devine, Edgar R. Gomes, Joseph Chambers, Elena Koslover, Edward Avezov   +13 more
wiley   +1 more source

Quantifying uncertainty in phasor-based time-domain fluorescence lifetime imaging microscopy. [PDF]

Biomed Opt Express
Chen Q, Park J, Mu S, Gao L.
europepmc   +1 more source

Persistently Increased Expression of PKMzeta and Unbiased Gene Expression Profiles Identify Hippocampal Molecular Traces of a Long‐Term Active Place Avoidance Memory and “Shadow” Proteins

Advanced Science, EarlyView.
Protein complexes like KIBRA‐PKMζ are crucial for maintaining memories, forming month‐long protein traces in memory‐tagged neurons, but conventional RNA‐seq analysis fails to detect their transcript changes, leaving memory molecules undetected in the shadows of abundantly‐expressed genes.
Jiyeon Han, Alejandro Grau‐Perales, Rayna M. Harris, Edith Lesburguères, Hsin‐Yi Kao, Asit Pal, Juan Marcos Alarcon, Todd C. Sacktor, Stefano Martiniani, Hans A. Hofmann, André A. Fenton   +10 more
wiley   +1 more source

Fluorescence lifetime imaging microscopy approach reveals quantitative signatures for hepatocellular carcinoma diagnosis. [PDF]

Front Oncol
Li L, Wang A, Liu X, Wang G, Xu S, Li C, Wu W, Zhang X, He Z, Qiu H, Peng X, Yan W, Qu J.   +12 more
europepmc   +1 more source

Visualizing orthogonal RNAs simultaneously in live mammalian cells by fluorescence lifetime imaging microscopy (FLIM). [PDF]

Nat Commun, 2023
Sarfraz N, Moscoso E, Oertel T, Lee HJ, Ranjit S, Braselmann E.   +5 more
europepmc   +1 more source

First Generation Proteolysis Targeting Chimeras (PROTACs) for the Treatment of Progeria

Advanced Science, EarlyView.
We report the first PROTACs designed to degrade progerin, introducing a novel therapeutic approach for progeria. The best compound, UCM‐18142, significantly reduces progerin levels and improves key disease phenotypes in patient‐derived cells and in the LmnaG609G/G609G mouse model, paving the way for new treatment strategies targeting the root cause of ...
Jon Macicior‐Michelena, Marcelino Telechea, Daniel Fernández, Alba García‐Martín, Ángeles Canales, Silvia Ortega‐Gutiérrez   +5 more
wiley   +1 more source

FLIMPA: A Versatile Software for Fluorescence Lifetime Imaging Microscopy Phasor Analysis. [PDF]

Anal Chem
Kapsiani S, Läubli NF, Ward EN, Shehata M, Kaminski CF, Kaminski Schierle GS.   +5 more
europepmc   +1 more source