An open-source, high-resolution, automated fluorescence microscope [PDF]
Fluorescence microscopy is a fundamental tool in the life sciences, but the availability of sophisticated equipment required to yield high-quality, quantitative data is a major bottleneck in data production in many laboratories worldwide.
Ando Christian Zehrer +3 more
doaj +4 more sources
Multimodal fluorescence microscope with fast adaptive polarimetry [PDF]
Polarized light microscopy is a widely used technique to observe specimens that are optically anisotropic, or birefringent. It has a broad applicability in the study of minerals, soft materials such as polymers, complex fluids or liquid crystals, and ...
Berta Martínez-Prat +3 more
doaj +5 more sources
Possibilities of using a miniature fluorescence microscope [PDF]
The article discusses an approach to fluorescence microscopy using single-photon miniature fluorescence microscopes. The use of miniscopes allows you to expand the possibilities of their usage and solve many problems related to the need of fixing ...
Erofeev Alexander +4 more
doaj +2 more sources
A miniature fluorescence microscope for multi-plane imaging [PDF]
Miniature fluorescence microscopes are becoming an increasingly established tool to investigate neural circuits in freely moving animals. In this work we present a lightweight one-photon microscope capable of imaging at different focal depths.
Giovanni Barbera +5 more
doaj +2 more sources
Fluorescence microscope light source based on integrated LED [PDF]
An LED-integrated excitation cube (LEC) was designed to address the limitations of conventional fluorescence lamps. The LEC has a decentralized structure, high optical power density, and efficient illumination.
Jianchen Zi, Hai Bi
doaj +2 more sources
A Smartphone-Based Low-Cost Inverted Laser Fluorescence Microscope for Disease Diagnosis [PDF]
Fluorescence microscopy is an important tool for disease diagnosis, often requiring costly optical components, such as fluorescence filter cubes and high-power light sources.
Omar Ormachea +3 more
doaj +2 more sources
A cryogenic, coincident fluorescence, electron, and ion beam microscope [PDF]
Cryogenic electron tomography (cryo-ET) combined with subtomogram averaging, allows in situ visualization and structure determination of macromolecular complexes at subnanometre resolution.
Daan B Boltje +15 more
doaj +2 more sources
Automated microscope-independent fluorescence-guided micropipette
Glass micropipette electrodes are commonly used to provide high resolution recordings of neurons. Although it is the gold standard for single cell recordings, it is highly dependent on the skill of the electrophysiologist. Here, we demonstrate a method of guiding micropipette electrodes to neurons by collecting fluorescence at the aperture, using an ...
Christopher Miranda +6 more
openaire +3 more sources
Low-cost fluorescence microscope with microfluidic device fabrication for optofluidic applications [PDF]
Optofluidic devices have revolutionized the manipulation and transportation of fluid at smaller length scales ranging from micrometers to millimeters. We describe a dedicated optical setup for studying laser-induced cavitation inside a microchannel. In a
Nagaraj Nagalingam +8 more
doaj +2 more sources
Homologous Recombination under the Single-Molecule Fluorescence Microscope. [PDF]
Homologous recombination (HR) is a complex biological process and is central to meiosis and for repair of DNA double-strand breaks. Although the HR process has been the subject of intensive study for more than three decades, the complex protein–protein ...
Gibbs DR, Dhakal S.
europepmc +3 more sources

