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Properties of a 4Pi confocal fluorescence microscope
Journal of the Optical Society of America A, 1992 In a 4Pi confocal fluorescence microscope two opposing microscope objective lenses were used to illuminate a fluorescent object from both sides and to collect the fluorescence emissions on both sides. Constructive interference of either the illumination wave fronts in the common focus or the detection wave fronts in the common detector pinhole resulted
Hell, Stefan, Stelzer, E. H. K .
openaire +3 more sources
Standing-wave-excited multiplanar fluorescence in a laser scanning microscope reveals 3D information on red blood cells [PDF]
, 2014 Standing-wave excitation of fluorescence is highly desirable in optical microscopy because it improves the axial resolution. We demonstrate here that multiplanar excitation of fluorescence by a standing wave can be produced in a single-spot laser ...
Amor, Rumelo +3 more
core +2 more sources
FEBS Letters, EarlyView.The protonated form of butyrate, as well as other short‐chain fatty acids (SCFAs), is membrane permeable. In acidic extracellular environments, this can lead to intracellular accumulation of SCFAs and cytosolic acidification. This phenomenon will be particularly relevant in acidic environments such as the large intestine or tumor microenvironments ...
Muwei Jiang +2 more
wiley +1 more source
Centimeter-deep tissue fluorescence microscopic imaging with high signal-to-noise ratio and picomole sensitivity [PDF]
, 2015 Fluorescence microscopic imaging in centimeter-deep tissue has been highly sought-after for many years because much interesting in vivo micro-information, such as microcirculation, tumor angiogenesis, and metastasis, may deeply locate in tissue. In this study, for the first time this goal has been achieved in 3-centimeter deep tissue with high signal ...
arxiv +1 more source
Ultrawidefield microscope for high-speed fluorescence imaging and targeted optogenetic stimulation.
Biomedical Optics Express, 2017 The rapid increase in the number and quality of fluorescent reporters and optogenetic actuators has yielded a powerful set of tools for recording and controlling cellular state and function.
C. Werley, Miao-Ping Chien, A. Cohen
semanticscholar +1 more source
Planar Airy beam light-sheet for two-photon microscopy [PDF]
, 2020 We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-
Almada, Pedro +12 more
core +3 more sources
Molecular Oncology, EarlyView.Cancer‐associated fibroblasts (CAFs) promote cancer growth, invasion (metastasis), and drug resistance. Here, we identified functional and diverse circulating CAFs (cCAFs) in patients with metastatic prostate cancer (mPCa). cCAFs were found in higher numbers and were functional and diverse in mPCa patients versus healthy individuals, suggesting their ...
Richell Booijink +6 more
wiley +1 more source
Spatially resolved coherent 2D fluorescence spectroscopy within a high-NA microscope [PDF]
EPJ Web of Conferences, 2019 We have developed coherent two-dimensional (2D) fluorescence micro-spectroscopy which probes the nonlinear optical response at surfaces via fluorescence detection with sub-micron spatial resolution.
Li Donghai +7 more
doaj +1 more source
A novel Ca2+ indicator for long-term tracking of intracellular calcium flux
BioTechniques, 2021 The major drawback of using Fluo-4 AM is that it requires an organic anion transporter inhibitor, such as probenecid, to prevent leakage. This can hinder the studies that require extended monitoring time and longer cellular retention.
Jinfang Liao +5 more
doaj +1 more source
Fluorescence Microscope: A Review Article [PDF]
, 2018 A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence to study properties of organic or inorganic substances.
Bihonegn, Temesgen
core +1 more source