Results 291 to 300 of about 1,071,713 (341)
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Study of microscopic porphyrin fluorescence
Urology, 1987A technique of porphyrin (hematoporphyrin derivative [Hpd]) fluorescence microscopy was evaluated by studying Hpd uptake, retention, and loss in established human cancer cell lines. Hpd uptake appeared to be qualitatively identical in the three cell lines used, but the rate of loss was slowest in the renal-carcinoma-derived line, suggesting a cellular ...
L D, Rosenstein, U O, Nseyo, J E, Pontes
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Microscopic fluorescence in photodynamic therapy
The Laryngoscope, 1986AbstractThe use of hematoporphyrin derivative (HPD) as a photosensitizer has been studied in malignant tumor detection via fluorescence and in tumor destruction via a toxic photochemical reaction. Squamous cell carcinoma has been induced in hamster buccal mucosa utilizing the known carcinogen dimethyl‐benzanthracene. HPD was injected intraperitoneally,
L F, Berg, D M, Harris
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Laser Scanning Fluorescence Microscope
SPIE Proceedings, 1988We report on the development of a laser scanning fluorescence microscope possessing several features which facilitate its application to biological and biophysical analyses in living cells. It is built around a standard inverted microscope stand, enabling the use of standard optics, micromanipulation apparatus, and conventional (including video ...
Eric W. Hansen +2 more
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Vidicon Microscope for Counting Fluorescent Particles
Review of Scientific Instruments, 1971Weakly fluorescent particles may be counted or displayed through use of the light integrating property of vidicons. This paper describes a fluorescence microscope-vidicon system designed for counting bacteria. It employs incident bright-field uv illumination with an inexpensive television (TV) system.
D L, Dyer, C H, Fuller
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Fluorescence in the tandem scanning microscope
Journal of Microscopy, 1990SUMMARYOur studies have shown that the fluorescence mode can be used to good effect in both tandem scanning microscopes (TSM: direct view confocal microscopes) as well as confocal scanning laser microscopes (CSLM). Applications are presented which show that the two great advantages of TSM are real‐time viewing and real colour, which allow faster use ...
A, Boyde +4 more
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Troubleshooting the Fluorescence Microscope
Laboratory Medicine, 1980The fluorescence microscope is being used increasingly in clinical laboratories as a diagnostic tool. A troubleshooting guide for the fluorescence microscope is presented to aid laboratorians in investigating system malfunctions.
Ronald P. Neimeister +3 more
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Bayesian Tracking for Fluorescence Microscopic Imaging
3rd IEEE International Symposium on Biomedical Imaging: Macro to Nano, 2006., 2006Fluorescence microscopy is a powerful imaging tool for studying molecular dynamics in living cells. For quantitative motion analysis of subcellular structures robust and accurate detection and tracking techniques are necessary sequential Monte Carlo methods, also known as particle filters (PF), have become a tremendously popular tool to perform ...
Smal, Ihor +2 more
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Femtosecond Fluorescence Dynamics Imaging Using a Fluorescence Up-Conversion Microscope
The Journal of Physical Chemistry B, 2005Femtosecond fluorescence dynamics imaging microscopy was performed. Femtosecond fluorescence dynamics images were constructed based on the "mean" fluorescence decay or rise time constants that were evaluated by the time-resolved intensity sampling using a fluorescence up-conversion microscope.
Tatsuya, Fujino +2 more
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Evaluating the performance of fluorescence microscopes
Journal of Microscopy, 1998A simple means of evaluating the performance of fluorescence microscopes is described. The proposed test gives an overall figure of merit that takes into account all of the important instrumental parameters that determine image quality. The essence of the test is to use a specimen whose photobleaching rate is a measure of the illumination time ...
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Fluorescence microscopical hybridocytochemistry.
Acta histochemica. Supplementband, 1985A new method has been developed to detect RNA-DNA hybrids in situ by fluorescence microscopy. This overcomes some of the disadvantages of autoradiographical detection of in situ hybridization, notably the low resolution and long exposure times needed. A procedure to label RNA at its 3'-terminus with a fluorochrome molecule has been developed.
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