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Troubleshooting the Fluorescence Microscope
Laboratory Medicine, 1980The fluorescence microscope is being used increasingly in clinical laboratories as a diagnostic tool. A troubleshooting guide for the fluorescence microscope is presented to aid laboratorians in investigating system malfunctions.
Vern Pidcoe +3 more
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Evaluating the performance of fluorescence microscopes
Journal of Microscopy, 1998A simple means of evaluating the performance of fluorescence microscopes is described. The proposed test gives an overall figure of merit that takes into account all of the important instrumental parameters that determine image quality. The essence of the test is to use a specimen whose photobleaching rate is a measure of the illumination time ...
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Laser Scanning Fluorescence Microscope
SPIE Proceedings, 1988We report on the development of a laser scanning fluorescence microscope possessing several features which facilitate its application to biological and biophysical analyses in living cells. It is built around a standard inverted microscope stand, enabling the use of standard optics, micromanipulation apparatus, and conventional (including video ...
Benjamin A. Wiseman +2 more
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Quantitative determination of mitophagy by fluorescence microscope
2021Mitophagy is an evolutionally conserved cellular process that eliminates dysfunctional and excess mitochondria, thereby facilitating mitochondrial quality control and metabolic recycling. In addition, mitophagy is essential for cellular homeostasis and tissue development, and mitophagic dysfunction is related to various pathologies including ...
Qi Wu +5 more
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Vidicon Microscope for Counting Fluorescent Particles
Review of Scientific Instruments, 1971Weakly fluorescent particles may be counted or displayed through use of the light integrating property of vidicons. This paper describes a fluorescence microscope-vidicon system designed for counting bacteria. It employs incident bright-field uv illumination with an inexpensive television (TV) system.
D. L. Dyer, C. H. Fuller
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A Guide to Purchasing a Fluorescence Microscope
Laboratory Medicine, 1981When purchasing a fluorescence microscope to fulfill the needs of the laboratory, it is imperative that one consider the following factors before making a .final decision: the procedures to be performed; the type of microscope that would best perform these procedures; the interaction of the individual components of the microscope; the total cost of the
Josephine Bartola, Ron Neimeister
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Fluorescence Microscopic Methods in Histochemistry
1991The majority of tissue components in unstained untreated sections show some degree of fluorescence. This is called primary fluorescence or autofluorescence. Such autofluorescence is particularly pronounced in plant tissues, while in animal tissue, collagen, elastin, and lipofuscin are noted for this property.
H. Lyon, M. Møller
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High-speed atomic force microscope combined with single-molecule fluorescence microscope.
Review of Scientific Instruments, 2013High-speed atomic force microscopy (HS-AFM) and total internal reflection fluorescence microscopy (TIRFM) have mutually complementary capabilities. Here, we report techniques to combine these microscopy systems so that both microscopy capabilities can be
Shingo Fukuda +6 more
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Configuration of a Fluorescence Microscope
2010This excerpt gives a succinct explanation of the configuration of a fluorescence microscope.
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