Results 41 to 50 of about 1,071,713 (341)
A novel Ca2+ indicator for long-term tracking of intracellular calcium flux
BioTechniques, 2021 The major drawback of using Fluo-4 AM is that it requires an organic anion transporter inhibitor, such as probenecid, to prevent leakage. This can hinder the studies that require extended monitoring time and longer cellular retention.
Jinfang Liao +5 more
doaj +1 more source
Single-atom imaging of fermions in a quantum-gas microscope [PDF]
, 2015 Single-atom-resolved detection in optical lattices using quantum-gas microscopes has enabled a new generation of experiments in the field of quantum simulation.
Andrew Kelly +39 more
core +3 more sources
A Workingperson’s Guide to Deconvolution in Light Microscopy
BioTechniques, 2001 The fluorescence microscope is routinely used to study cellular structure in many biomedical research laboratories and is increasingly used as a quantitative assay system for cellular dynamics.
Wes Wallace +2 more
doaj +1 more source
Integrated photoacoustic, confocal, and two-photon microscope [PDF]
, 2014 The invention of green fluorescent protein and other molecular fluorescent probes has promoted applications of confocal and two-photon fluorescence microscopy in biology and medicine.
Kerschensteiner, Daniel +3 more
core +1 more source
Fluorescence advantages with microscopic spatiotemporal control [PDF]
SPIE Proceedings, 2013 We present a clever design concept of using femtosecond laser pulses in microscopy by selective excitation or de-excitation of one fluorophore over the other overlapping one. Using either a simple pair of femtosecond pulses with variable delay or using a train of laser pulses at 20-50 Giga-Hertz excitation, we show controlled fluorescence excitation or
Debabrata, Goswami +2 more
openaire +2 more sources
Microfabricated rubber microscope using soft solid immersion lenses [PDF]
, 2006 We show here a technique of soft lithography to microfabricate efficient solid immersion lenses (SIL) out of rubber elastomers. The light collection efficiency of a lens system is described by its numerical aperture (NA), and is critical for applications
Gambin, Yann +2 more
core +1 more source
A fluorescence scanning electron microscope
Ultramicroscopy, 2009 Fluorescence techniques are widely used in biological research to examine molecular localization, while electron microscopy can provide unique ultrastructural information. To date, correlative images from both fluorescence and electron microscopy have been obtained separately using two different instruments, i.e.
Kanemaru, Takaaki +6 more
openaire +2 more sources
Fluorescence recovery after photobleaching on the confocal laser-scanning microscope: generalized model without restriction on the size of the photobleached disk [PDF]
, 2011 Fluorescence recovery after photobleaching (FRAP) carried out on a confocal laser-scanning microscope (CLSM) performs well for photobleached disks that are large compared to the resolution of the bleaching beam.
Ameloot, Marcel +6 more
core +2 more sources
An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots
FEBS Letters, EarlyView.This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino +7 more
wiley +1 more source
Biosensors, 2022 The fluorescence microscope has been widely used to explore dynamic processes in vivo in mouse brains, with advantages of a large field-of-view and high spatiotemporal resolution.
Rujin Zhang +9 more
doaj +1 more source