Results 41 to 50 of about 1,065,070 (295)

Fluorescence recovery after photobleaching on the confocal laser-scanning microscope: generalized model without restriction on the size of the photobleached disk [PDF]

, 2011
Fluorescence recovery after photobleaching (FRAP) carried out on a confocal laser-scanning microscope (CLSM) performs well for photobleached disks that are large compared to the resolution of the bleaching beam.
Ameloot, Marcel, Braeckmans, Kevin, De Smedt, Stefaan, Deschout, Hendrik, Rigo, Jean-Michel, Smisdom, Nick, vandeVen, Martin   +6 more
core   +2 more sources

Fluorescence advantages with microscopic spatiotemporal control [PDF]

SPIE Proceedings, 2013
We present a clever design concept of using femtosecond laser pulses in microscopy by selective excitation or de-excitation of one fluorophore over the other overlapping one. Using either a simple pair of femtosecond pulses with variable delay or using a train of laser pulses at 20-50 Giga-Hertz excitation, we show controlled fluorescence excitation or
Debabrata, Goswami, Debjit, Roy, Arijit K, De   +2 more
openaire   +2 more sources

Automated microscope-independent fluorescence-guided micropipette

Biomedical Optics Express, 2021
Glass micropipette electrodes are commonly used to provide high resolution recordings of neurons. Although it is the gold standard for single cell recordings, it is highly dependent on the skill of the electrophysiologist. Here, we demonstrate a method of guiding micropipette electrodes to neurons by collecting fluorescence at the aperture, using an ...
Christopher Miranda, Madeleine R. Howell, Joel F. Lusk, Ethan Marschall, Jarrett Eshima, Trent Anderson, Barbara S. Smith   +6 more
openaire   +2 more sources

A fluorescence scanning electron microscope

Ultramicroscopy, 2009
Fluorescence techniques are widely used in biological research to examine molecular localization, while electron microscopy can provide unique ultrastructural information. To date, correlative images from both fluorescence and electron microscopy have been obtained separately using two different instruments, i.e.
Kanemaru, Takaaki, Hirata, Kazuho, Takasu, Shin-ichi, Isobe, Shin-ichiro, Mizuki, Keiji, Mataka, Shuntaro, Nakamura, Kei-ichiro   +6 more
openaire   +2 more sources

Simultaneous Observation of Mouse Cortical and Hippocampal Neural Dynamics under Anesthesia through a Cranial Microprism Window

Biosensors, 2022
The fluorescence microscope has been widely used to explore dynamic processes in vivo in mouse brains, with advantages of a large field-of-view and high spatiotemporal resolution.
Rujin Zhang, Chaowei Zhuang, Zilin Wang, Guihua Xiao, Kunsha Chen, Hao Li, Li Tong, Weidong Mi, Hao Xie, Jiangbei Cao   +9 more
doaj   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Miniaturized integration of a fluorescence microscope [PDF]

Nature Methods, 2011
The light microscope is traditionally an instrument of substantial size and expense. Its miniaturized integration would enable many new applications based on mass-producible, tiny microscopes. Key prospective usages include brain imaging in behaving animals for relating cellular dynamics to animal behavior.
Ghosh, Kunal K., Burns, Laurie D., Cocker, Eric D., Nimmerjahn, Axel, Ziv, Yaniv, Gamal, Abbas El, Schnitzer, Mark J.   +6 more
openaire   +2 more sources

Microfabricated rubber microscope using soft solid immersion lenses [PDF]

, 2006
We show here a technique of soft lithography to microfabricate efficient solid immersion lenses (SIL) out of rubber elastomers. The light collection efficiency of a lens system is described by its numerical aperture (NA), and is critical for applications
Gambin, Yann, Legrand, Olivier, Quake, Stephen R.   +2 more
core   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Integrated photoacoustic, confocal, and two-photon microscope [PDF]

, 2014
The invention of green fluorescent protein and other molecular fluorescent probes has promoted applications of confocal and two-photon fluorescence microscopy in biology and medicine.
Kerschensteiner, Daniel, Rao, Bin, Soto, Florentina, Wang, Lihong V.   +3 more
core   +1 more source