Results 51 to 60 of about 1,071,713 (341)

Miniaturized integration of a fluorescence microscope [PDF]

Nature Methods, 2011
The light microscope is traditionally an instrument of substantial size and expense. Its miniaturized integration would enable many new applications based on mass-producible, tiny microscopes. Key prospective usages include brain imaging in behaving animals for relating cellular dynamics to animal behavior.
Ghosh, Kunal K., Burns, Laurie D., Cocker, Eric D., Nimmerjahn, Axel, Ziv, Yaniv, Gamal, Abbas El, Schnitzer, Mark J.   +6 more
openaire   +2 more sources

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

Fast wavelength-tunable ultra-violet laser source for confocal Fura-2AM imaging [PDF]

, 2007
We report a novel wavelength-flexible laser source for three-dimensional ultra-violet imaging. Based on supercontinuum generation in photonic crystal fiber, the resultant broadband laser source extended from A = 331 nm into the visible region of the ...
Esposito, E., Harris, J., McConnell, G.
core   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Automatic Slide-Loader Fluorescence Microscope for Discriminative Enumeration of Subseafloor Life [PDF]

Scientific Drilling, 2010
No abstract available.
Y. Morono, F. Inagaki
doaj   +3 more sources

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Evaluating Different Counter Stains in Fluorescent Staining Technique for Detecting Acid Fast Bacilli: Best Amongst The Better

Journal of Pure and Applied Microbiology, 2018
Tuberculosis is a global emergency needing an early and accurate diagnosis for management and control. Smear microscopy is a rapid diagnostic method for detecting acid fast bacilli (AFB) and improvising this method would contribute in better detection ...
Shameema Mol N, Vishnu Prasad Shenoy, Seema Shetty, Kiran Chawla   +3 more
doaj   +1 more source

Some Secrets of Fluorescent Proteins: Distinct Bleaching in Various Mounting Fluids and Photoactivation of cyan fluorescent proteins at YFP-Excitation [PDF]

, 2011
Background The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or yellow mutants (CFP and YFP, respectively) is very common in all different fields of life sciences, e.g. for marking specific proteins or cells
AF Fradkov, AG Harpur, Andreas Hofmann, C Dinant MEVRWVABH, C Thaler, DW Piston, EA Jares-Erijman, EB Van Munster, FS Wouters, FS Wouters, G Valentin, H-w Ai, J Goedhart, JA Schmid, JA Schmid, JA Schmid, JM Lingbeck, Johannes A. Schmid, K Ebner, L Abrami, M Ormo, MA Rizzo, MK Raarup, MT Kirber, Naila Malkani, NC Shaner, PI Bastiaens, PI Bastiaens, RY Tsien, SE Verrier, T Karpova, T Nagai, TB McAnaney, TW Gadella Jr   +33 more
core   +4 more sources

Cell density–dependent nuclear‐cytoplasmic shuttling of SETDB1 integrates with Hippo signaling to regulate YAP1‐mediated transcription

FEBS Letters, EarlyView.
At low cell density, SETDB1 and YAP1 accumulate in the nucleus. As cell density increases, the Hippo pathway is gradually activated, and SETDB1 is associated with increased YAP1 phosphorylation. At high cell density, phosphorylated YAP1 is sequestered in the cytoplasm, while SETDB1 becomes polyubiquitinated and degraded by the ubiquitin–proteasome ...
Jaemin Eom, Jaewoong Jang, Jung Sun Park, Yong‐Kook Kang   +3 more
wiley   +1 more source

Fabrication of Bioactive Surfaces by Functionalization of Electroactive and Surface-Active Block Copolymers

Bioengineering, 2014
Biofunctional block copolymers are becoming increasingly attractive materials as active components in biosensors and other nanoscale electronic devices.
Omotunde Olubi, Laurisa London, Biswajit Sannigrahi, Peri Nagappan, Michael Williams, Ishrat M. Khan   +5 more
doaj   +1 more source