Results 121 to 130 of about 769,414 (303)

Modern age of pathological sciences: Fluorescence microscopy for proficient treatment

In light microscopy, achieving uniform lighting throughout the field of view is essential to obtain clear images. However, thicker samples commonly suffer from image blur due to out-of-focus illumination, notably raised in fluorescence microscopy because
Mehmood, Muhammad Qasim, Zubair, Muhammad, Javed, Isma, Satti, Azhar Javed   +3 more
core   +1 more source

2-Photon Laser Scanning Microscopy on Native Human Cartilage

, 2005
Martini J, Tönsing K, Dickob M, Anselmetti D. 2-Photon Laser Scanning Microscopy on Native Human Cartilage. In: Proceedings of SPIE - The International Society for Optical Engineering. Vol 5860.
Michael Dickob, Martini, Jörg, Joerg Martini, Dickob, Michael, Katja Toensing, Dario Anselmetti, Tönsing, Katja, Anselmetti, Dario ; https://orcid.org/   +7 more
core   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Evaluation of combined LED-fluorescence microscopy and bleach sedimentation for diagnosis of tuberculosis at peripheral health service level.

, 2011
Sputum microscopy is the only diagnostic for tuberculosis (TB) available at peripheral levels of health service in resource-poor countries. Its sensitivity is reduced in high HIV-prevalence settings.
Gagnidze Laramie, Gagnidze, Laramie, Guerin, P J, Maryline Bonnet, Philippe J Guerin, Ramsay, A, Guerin, Philippe J, Ramsay Andrew, Bonnet, M, Gagnidze, L, Francis Varaine, Guerin Philippe J., Githui, W, Guerin, PJ, Githui Willie, Bonte, L, Bonte, Laurence, Varaine, Francis, Philippe J. Guerin, Bonte Laurence, Laramie Gagnidze, Bonnet, Maryline, Varaine, F, Varaine Francis, Willie Githui, Laurence Bonte, Andrew Ramsay, Guerin, Philippe, Ramsay, Andrew, Githui, Willie, Bonnet Maryline   +30 more
core   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Fluorescence microscopy in the spotlight [PDF]

Microscopy Research and Technique, 2014
Francesca Cella, Zanacchi, Paolo, Bianchini, Giuseppe, Vicidomini   +2 more
openaire   +2 more sources

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source