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Fluorescence microscopy: strategic blinking.
Nature chemistry, 2014For decades chemists have focused on increasing the brightness of fluorophores. In super-resolution microscopy, however, fluorophores that preferentially exist in a non-fluorescent state, but occasionally re-arrange into a fluorescent form, can give better results.
Lukinavicius, Grazvydas, Johnsson, Kai
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Digital imaging fluorescence microscopy
1986For digital imaging fluorescence microscopy we have used a Grinnell 274 image processor interfaced to a Lab Datex LSI 11/23 under RT-11 controlled by Integrated Solutions 68010 operating under Unix BSD 4.2. Experimental approaches to address (a) photobleaching of the fluorophore, (b) misalignment of images by nonparfocal microscope optics and (c) the ...
Smith, L.C. +3 more
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2005
Fluorescence microscopy is the essential technique for investigation of the intracellular distribution of macromolecules and various organelles also in yeast cells. In this chapter, detailed practical procedures for fluorescence microscopic observations developed or adopted in our laboratory are described.
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Fluorescence microscopy is the essential technique for investigation of the intracellular distribution of macromolecules and various organelles also in yeast cells. In this chapter, detailed practical procedures for fluorescence microscopic observations developed or adopted in our laboratory are described.
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Fluorescence Microscopy; Quantitative Fluorescence Microscopy
Systematic Biology, 1993openaire +1 more source
Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations
Cells, 2022Joanna Lazniewska +2 more
exaly
Confocal Fluorescence-Lifetime Single-Molecule Localization Microscopy
ACS Nano, 2020Jörg Enderlein +2 more
exaly