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Open-Source 3D Active Sample Stabilization for Fluorescence Microscopy
Patil S, Vicidomini G, Slenders E.
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Fluorescence tissue microscopy
Progress in Biophysics and Molecular Biology, 2022The increasing need to investigate large biological specimens like whole tissues in 3D has driven the development of novel optical techniques capable of volumetric imaging with no compromises in terms of field-of-view and spatial resolution. On the other hand, a major challenge in the imaging of bulk biological tissues is their strong scattering which ...
Sacconi, L +3 more
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Nature Methods, 2005
Although fluorescence microscopy permeates all of cell and molecular biology, most biologists have little experience with the underlying photophysical phenomena. Understanding the principles underlying fluorescence microscopy is useful when attempting to solve imaging problems.
Jeff W, Lichtman, José-Angel, Conchello
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Although fluorescence microscopy permeates all of cell and molecular biology, most biologists have little experience with the underlying photophysical phenomena. Understanding the principles underlying fluorescence microscopy is useful when attempting to solve imaging problems.
Jeff W, Lichtman, José-Angel, Conchello
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Fluorescent Speckle Microscopy
Cold Spring Harbor Protocols, 2011INTRODUCTIONFluorescent speckle microscopy (FSM) is a live imaging and quantitative measurement technique used for analyzing motion and turnover of macromolecular assemblies in vivo and in vitro. It differs from related imaging techniques such as photobleaching and photoactivation in its use of substantially lower concentrations of fluorescently ...
Lisa A, Cameron +2 more
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Current Protocols in Immunology, 1998
AbstractAbsorption of energy as light by some molecules and emission of energy as fluorescence can occur only at certain wavelengths, which are characteristic for a given molecule (fluorophore). Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific molecules and analyzed cellular ...
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AbstractAbsorption of energy as light by some molecules and emission of energy as fluorescence can occur only at certain wavelengths, which are characteristic for a given molecule (fluorophore). Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific molecules and analyzed cellular ...
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2019
In this two-part chapter, the background to confocal microscopy and two-photon fluorescence microscopy is first presented, with a detailed description of the optical setup. This is followed by a critical account of the many super-resolution techniques: coordinated stochastic fluorescence microscopy (photoactivation localization microscopy ...
Alberto Diaspro +7 more
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In this two-part chapter, the background to confocal microscopy and two-photon fluorescence microscopy is first presented, with a detailed description of the optical setup. This is followed by a critical account of the many super-resolution techniques: coordinated stochastic fluorescence microscopy (photoactivation localization microscopy ...
Alberto Diaspro +7 more
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Fluorescence (Multiwave) Confocal Microscopy
Dermatologic Clinics, 2016In addition to reflectance confocal microscopy, multiwave confocal microscopes with different laser wavelengths in combination with exogenous fluorophores allow fluorescence mode confocal microscopy in vivo and ex vivo. Fluorescence mode confocal microscopy improves the contrast between the epithelium and the surrounding soft tissue and allows the ...
Welzel, Julia +2 more
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Nature Methods, 2005
Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues.
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Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues.
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