Results 111 to 120 of about 3,402,841 (337)

Emerging experimental and computational methods for studying redox‐regulated structural transitions

FEBS Letters, EarlyView.
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass, Dana Reichmann, Gábor Erdős   +2 more
wiley   +1 more source

Synthesis and properties of new fluorescent dyes and dye-labelled oligonucleotides

, 2012
Fluorescence is widely used in DNA sequencing and genetic analysis. Novel fluorescence techniques for studying nucleic acids and other biologically important molecules are constantly being developed.
Gerowska, Marta
core   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Translophagy—A potential link between autophagy impairment and translational errors

FEBS Letters, EarlyView.
Neurodegenerative diseases are characterised by the accumulation of abnormal proteins and protein aggregates, but their origin often remains unknown. We propose that selective autophagy removes damaged protein‐making machinery, preventing errors during protein synthesis.
Mykola V. Korolchuk, Gailing Ma, Robin Antrobus, Diego Manni, Marco Trevisan‐Herraz, Mustafa Khwaja, Max Brown, Gavin D. Richardson, Masaaki Komatsu, Daniel Erskine, Viktor I. Korolchuk, Tetsushi Kataura   +11 more
wiley   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Technical Note: Determination of the metabolically active fraction of benthic foraminifera by means of Fluorescent In Situ Hybridization (FISH) [PDF]

, 2011
Benthic foraminifera are an important component of the marine biota, but protocols for investigating their viability and metabolism are still extremely limited.
Negri, Alessandra, Danovaro, R., Luna, G, M, Nardelli, M., Negri A., Morigi, C., C. Morigi, Sabbatini A, Nardelli MP, Borrelli, C, Sabbatini, A., Borrelli C, Luna GM, Luna, Gian Marco, Sbaffi, T, M.P. Nardelli, Danovaro R, R. Danovaro, A. Sabbatini, G.M. Luna, Borrelli, Chiara, Luna, G,, Sabbatini, Anna, Negri, A, M. P. Nardelli, Nardelli, Maria Pia, Morigi, Caterina, Danovaro, Roberto, A. Negri, G. M. Luna, T. Sbaffi, C. Borrelli, Sbaffi T, Sbaffi, Tomasa   +33 more
core   +2 more sources

AI‐Driven Acceleration of Fluorescence Probe Discovery

Advanced Science
Fluorescence imaging probes are indispensable tools for clinical navigation and preclinical research. However, the discovery of target‐specific probes is hampered by the scarcity of targetable fluorophore scaffolds, making the development process slow ...
Xuefeng Jiang, Yanbo Li, Xue Tian, Sen Yang, Ruina Luo, Cenxing Zhou, Yuxuan Liu, Jingying Hu, Sen Feng, Lu Gan, Chongzhao Ran, Kun‐Hsing Yu, Junhan Zhao, Xiao Han, Xuan Zhai, Yuntao Jia, Jiapei Dai, Xiyue Wang, Biyue Zhu   +18 more
doaj   +1 more source

Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation

MethodsX, 2019
DNA-fluorescence in situ hybridisation (DNA-FISH) allows visualisation of chromosome organisation and rearrangement. FISH probes are pools of short fluorescently labelled DNA fragments that are often produced from template plasmids that contain large ...
Patrick J. McCoy, Anthony J. Costello, Niall M. Corcoran, Christopher M. Hovens, Michael J. Clarkson   +4 more
doaj   +1 more source

Invisible but not inaccessible—Revealing transient oligomers formed by intrinsically disordered proteins with solution NMR and complementary methods

FEBS Letters, EarlyView.
Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley   +1 more source

Wide-Field Multi-Parameter FLIM: Long-Term Minimal Invasive Observation of Proteins in Living Cells. [PDF]

, 2011
Time-domain Fluorescence Lifetime Imaging Microscopy (FLIM) is a remarkable tool to monitor the dynamics of fluorophore-tagged protein domains inside living cells.
Yury Prokazov, Turbin, E., Juan Llopis, Duci, A., Roland Hartig, Götze, C., Zuschratter, W., Christian Götze, Prokazov, Y., Hartig, R., Evgeny Turbin, Antonie J. W. G. Visser, Marco Vitali, Visser, A.J.W.G., Alessandro Duci, Werner Zuschratter, Vitali, M., Fernando Picazo, Picazo, F., Llopis, J., Antonie J W G Visser   +20 more
core   +2 more sources