Detection of Protein–Protein Interactions in Escherichia coli With Single Molecule Sensitivity
This article describes a regulatory circuit in Escherichia coli able to detect protein–protein interactions with exquisite sensitivity. The interaction between two hybrid proteins fused to Bordetella pertussis adenylate cyclase and its activator calmodulin triggers a potent cyclic AMP signaling cascade.
Marilyne Davi, Daniel Ladant
wiley +1 more source
Elucidating copper ion interactions with carbonic anhydrase: insights from fluorescence quenching and thermodynamic analysis. [PDF]
Shabanova MS +3 more
europepmc +1 more source
Critical assessment of selenourea as an efficient small molecule fluorescence quenching probe to monitor protein dynamics. [PDF]
Jena S +5 more
europepmc +1 more source
Fluorescence Studies of Photosystem II Fluorescence Quenching During Desiccation
Poikilohydric organisms have developed mechanisms to protect their photosynthetic machinery during times of desiccation. In hydrated conditions nonphotochemical quenching (NPQ) mechanisms are able to safely dissipate excess excitation energy as heat, but
Schaven, Kristin S.
core
Perivascular matrix densification promotes the emergence of aberrant endothelial tip cells (ATECs) that invade and persist within fibrotic microenvironments. Using in vivo lineage tracing and a human microvessel model, this study shows that fibrous matrix cues destabilize VE‐cadherin–mediated junctions to gate TGF‐β signaling, inducing a pro ...
Jingyi Xia +17 more
wiley +1 more source
Correction: A Rapid Fluorescence Quenching Assay for Total Levothyroxine Quantification in Pharmaceutical and Supratherapeutic Serum Samples. [PDF]
Abdel-Salam ET +3 more
europepmc +1 more source
Fluorescence quenching by high-power LEDs for highly sensitive fluorescence in situ hybridization. [PDF]
Tsuneoka Y +4 more
europepmc +1 more source
ERK‐Mediated Phosphorylation of YAP Defines a Noncanonical FGF Signaling Mechanism in Stem Cells
With a powerful combination of in vivo, ex vivo, and in vitro models, the authors highlight a novel FGF‐ERK signaling regulation of YAP at the S128 site in neural crest‐derived stem cells. This study opens exciting new directions in stem cell biology and craniofacial biology, paving the way for potential innovations in the treatment of craniofacial ...
Xiaolei Zhao +16 more
wiley +1 more source
Fluorescence Quenching Properties and Bioimaging Applications of Readily Accessible Blue to Far-Red Fluorogenic Triazinium Salts. [PDF]
Šlachtová V +9 more
europepmc +1 more source
Reply to Yan et al.: Quercetin possesses a fluorescence quenching effect but is a weak inhibitor against SARS-CoV-2 main protease. [PDF]
Xu H +6 more
europepmc +1 more source

