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Long-Term Fluorescence Recovery After Photobleaching (FRAP)
Methods in molecular biology, 2023Numerous models have been developed for the analysis of fluorescence recovery after photobleaching (FRAP), by which intracellular diffusion and turnover rate are quantitatively evaluated. FRAP analyses typically focus on such events that occur within several minutes, but to precisely evaluate a slow turnover rate of particularly actin stress fibers ...
Takumi, Saito +2 more
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Fluorescence Recovery after Photobleaching in Colloidal Science: Introduction and Application
ACS Biomaterials Science & Engineering, 2022FRAP (fluorescence recovery after photo bleaching) is a method for determining diffusion in material science. In industrial applications such as medications, foods, Medtech, hygiene, and textiles, the diffusion process has a substantial influence on the overall qualities of goods.
Aref Abbasi Moud
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Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis, and Applications
Methods in molecular biology, 2014A significant number of biological processes occur at, or involve cellular membranes, including; cell adhesion, migration, endocytosis, signal transduction, and many biochemical reactions involving membrane anchored scaffolds. Each process involves a complex arrangement of interacting molecules whose location in space and time influence the outcome of ...
Michael, Carnell +2 more
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Analysis of Protein Kinetics Using Fluorescence Recovery After Photobleaching (FRAP)
Methods in molecular biology, 2017Fluorescence recovery after photobleaching (FRAP) is a cutting-edge live-cell functional imaging technique that enables the exploration of protein dynamics in individual cells and thus permits the elucidation of protein mobility, function, and interactions at a single-cell level.
Nickolaos Nikiforos, Giakoumakis +2 more
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Fluorescence recovery after photobleaching
Imaging Modalities for Biological and Preclinical Research: A Compendium, Volume 1: Part I: Ex vivo biological imaging, 2021Favard, Cyril, Sibarita, Jean-Baptiste
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Novel Insights in Membrane Biology Utilizing Fluorescence Recovery After Photobleaching
Advances in Experimental Medicine and Biology, 2014Understanding cellular signaling by membrane receptors in terms of their lateral dynamics represents a challenging area in contemporary biology. Fluorescence recovery after photobleaching (FRAP) offers a convenient approach to measure lateral diffusion and is extensively used for measuring lateral diffusion of lipids and proteins in membranes.
Amitabha, Chattopadhyay, Md, Jafurulla
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Fluorescence Recovery After Photobleaching
2011This chapter describes the use of microscope-based fluorescence recovery after photobleaching (FRAP). To quantify the dynamics of proteins within a subcellular compartment, we first outline the general aspects of FRAP experiments and then provide a detailed protocol of how to measure and analyse the most important parameters of FRAP experiments such as
Carisey, Alex +3 more
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CM‐FRAP–Continuum Mechanics‐Based Fluorescence Recovery After Photobleaching
Current Protocols, 2023AbstractFluorescence recovery after photobleaching (FRAP) is widely used to evaluate intracellular molecular turnover or repeated translocation of molecules using confocal laser scanning microscopy. While numerous models have been developed for the analysis of FRAP responses, in which chemical interactions and/or fast diffusion processes are involved ...
Takumi, Saito, Shinji, Deguchi
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