Results 261 to 270 of about 262,308 (303)

Fluorescence Spectroscopy

2003
This chapter has introduced the theory of fluorescence, and briefly shown that it is a technique with many wide and varied applications in biochemistry. It has the overall advantage of being able to carry out measurements on small amounts of sample in solution, often revealing important information concerning the structure and dynamics of these systems.
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Fluorescence Spectroscopy of Peptides

2013
In peptides, steady-state fluorescence can be used to measure the intrinsic fluorescence of Trp, Tyr, and Phe residues, as well as associated dyes, which can change upon exposure to different environmental conditions. This technique can be thus used to detect changes in the conformational states of peptides.
Mangala R, Liyanage, Kunal, Bakshi, David B, Volkin, C Russell, Middaugh   +3 more
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Fluorescence Correlation Spectroscopy

2007
Fluorescence correlation spectroscopy (FCS) is a technique that allows for an extremely sensitive determination of molecular diffusion properties, down to the level of single molecules. It thus provides an attractive alternative to FRAP, requiring much less laser power and lower concentrations of fluorophores.
Kirsten, Bacia, Petra, Schwille
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Multimodal Fluorescence Imaging Spectroscopy

2013
Multimodal fluorescence imaging is a versatile method that has a wide application range from biological studies to materials science. Typical observables in multimodal fluorescence imaging are intensity, lifetime, excitation, and emission spectra which are recorded at chosen locations at the sample.
Stopel, M.H., Blum, C., Subramaniam, V., Subramaniam, V.   +3 more
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Fluorescence Correlation Spectroscopy

2014
Fluorescence fluctuation spectroscopy techniques allow the quantification of fluorescent molecules present at the nanomolar concentration level. After a brief introduction to the technique, this chapter presents a protocol including background information in order to measure and quantify the molecular interaction of two signaling proteins inside the ...
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Fluorescence Spectroscopy

Current Protocols Essential Laboratory Techniques, 2009
AbstractFluorescence is an extremely powerful tool in modern biology, physics, and chemistry laboratories. This unit begins with the physics of fluorescence, the biological applications of fluorescence, and the mechanisms behind spectrometers and fluorometers, followed by strategies to choose an appropriate fluorophore according to the instrumentation ...
Yin Yeh, Samantha Fore, Huawen Wu
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Television Spectroscopy of Biological Fluorescence

IRE Transactions on Medical Electronics, 1960
Fluorescence emission spectra were obtained from single unfixed ascites tumor cells stained at 3.3 × 10-7 M to 3.3 × 10-4 M concentrations of the vital fluorochrome, acridine orange. The new RCA Intensifier Image Orthicon television camera tube was used as the light detector and afforded approximately a one-hundredfold increase in sensitivity over a ...
S S, WEST, C N, LOESER, M D, SCHOENBERG
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Fluorescence spectroscopy

2005
Jiskoot, W., Visser, A.J.W.G., Herron, J.N., Sutter, M.   +3 more
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Fluorescence-Encoded Infrared Vibrational Spectroscopy with Single-Molecule Sensitivity

Journal of the American Chemical Society, 2021
Lukas Whaley-Mayda, Abhirup Guha, Andrei Tokmakoff   +2 more
exaly  

Fluorescence Spectroscopy

2019
Artur J. Moro, João Carlos Lima
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