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CYTOMEGALOVIRUS INFECTION: DETECTION BY DIRECT FLUORESCENT-ANTIBODY TECHNIQUE

The Lancet, 1972
Abstract A technique for detection of cytomegalovirus (C.M.V.) in tissue-culture by direct fluorescent-antibody staining is described. Of 150 urine specimens inoculated, 42 were positive by the fluorescent-antibody technique. By detection of cytopathic effect in routinely inoculated tissue culture, 36 of these were positive initially.
C H, Anderson, R H, Michaels
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Rabies neutralizing antibody determination in tissue culture by direct fluorescent antibody technique

Journal of Biological Standardization, 1975
Abstract Rabies neutralizing antibody levels were determined in 143 human serum samples by applying a direct fluorescent-antibody test to BHK-21/S13 cells treated previously with dilutions of the test samples mixed with constant amounts of challenge virus.
H C, Cho, P, Fenje
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Examination of Poultry Litter for Salmonellae by Direct Culture and Fluorescent Antibody Technique

Avian Diseases, 1976
Direct culture and fluorescent antibody (FA) procedures were used for examining approximately 6200 samples of poultry floor or nest litter for salmonellae. Each sample was cultured directly in tetrathionate brilliant-green (TBG) broth, incubated at 42 C for 48 hours, and plated on brilliant green (BG) agar.
C F, Smyser, G H, Snoeyenbos
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Usefulness of the Direct Fluorescent Antibody Technique for Diagnosis of Influenza in Swine

Journal of Veterinary Medicine, Series B, 1986
SummaryThe usefulness of the direct fluorescent antibody technique (FAT) for diagnosis of influenza in swine was determined. A total of 171 lung samples from 57 swine (3 samples per animal) were tested with both the direct FAT and virus isolation (VI) in embryonated eggs. A diagnosis of influenza was made in 16 swine.
M, Pensaert, F, Haesebrouck, F, Castryck
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Direct and indirect fluorescent-antibody staining techniques using commercial monoclonal antibodies for detection of respiratory syncytial virus

European Journal of Clinical Microbiology & Infectious Diseases, 1989
A comparison was made between direct and indirect fluorescent-antibody staining techniques using commercial monoclonal antibodies for detection of respiratory syncytial virus in respiratory secretions. Overall agreement between the two tests was 94%. Using virus isolation as the reference method, the indirect test had a higher sensitivity but a similar
C Y, Tong   +3 more
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Comparison of two direct fluorescent antibody techniques and cell culture for the detection of Chlamydia trachomatis

Serodiagnosis and Immunotherapy in Infectious Disease, 1990
Abstract Two direct immunofluorescence (DFA) techniques (Chlamydetect® and Microtrak®) and cell culture were compared for the detection of Chlamydia trachomatis in genital specimens from 200 patients attending a Sexually Transmitted Diseases clinic and 101 at a Gynecology clinic.
E.Ramirez de Arellano   +4 more
openaire   +1 more source

A Comparison of Cultural Methods used with Microcolony and Direct Fluorescent-Antibody Techniques to Detect Salmonellae

Journal of Milk and Food Technology, 1975
A comparison of various cultural methods for use with microcolony and direct fluorescent-antibody (FA) techniques to detect salmonella was conducted using 102 naturally contaminated samples. The methods were: (a) a 27-h microcolony technique, (b) a 51-h selenite-F FA microcolony technique, (c) a 51-h tetrathionate brilliant green FA microcolony ...
N. F. INSALATA   +2 more
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Detection of bovine enterotoxigenic Escherichia coli: A comparative study of a direct fluorescent antibody technique and conventional culturing methods

British Veterinary Journal, 1984
Abstract Experiments were conducted to evaluate the sensitivity and specificity of a direct fluorescent antibody technique (DFAT) in detecting bovine enterotoxigenic Escherichia coli possessing the K99 adherence antigen. A monospecific K99 antiserum was tested by DFAT against K99-positive and negative control strains and against faecal smears from ...
P, Lintermans, P, Pohl
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