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Digital imaging fluorescence microscopy
1986For digital imaging fluorescence microscopy we have used a Grinnell 274 image processor interfaced to a Lab Datex LSI 11/23 under RT-11 controlled by Integrated Solutions 68010 operating under Unix BSD 4.2. Experimental approaches to address (a) photobleaching of the fluorophore, (b) misalignment of images by nonparfocal microscope optics and (c) the ...
Smith, L.C. +3 more
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Multimodal Fluorescence Imaging Spectroscopy
2013Multimodal fluorescence imaging is a versatile method that has a wide application range from biological studies to materials science. Typical observables in multimodal fluorescence imaging are intensity, lifetime, excitation, and emission spectra which are recorded at chosen locations at the sample.
Stopel, M.H. +3 more
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FLUORESCENCE MOLECULAR IMAGING
Annual Review of Biomedical Engineering, 2006There is a wealth of new fluorescent reporter technologies for tagging of many cellular and subcellular processes in vivo. This imposed contrast is now captured with an increasing number of available imaging methods that offer new ways to visualize and quantify fluorescent markers distributed in tissues.
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Fluorescence Lifetime Imaging Microscopy
Current Protocols in Cell Biology, 2004AbstractFluorescent lifetime imaging microscopy is a powerful tool to enhance the contrast in images of biological samples and to investigate the local environment of a fluorochrome. FLIM allows the detection of protein‐protein interactions and their biochemical state by the quantitative detection of Förster resonance energy transfer (FRET) between ...
Alessandro, Esposito, Fred S, Wouters
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Two-Photon Fluorescence Imaging
2021Two-photon fluorescence imaging is a powerful tool for observing the dynamics of cells in vivo in intact tissue and is well suitable for imaging neuronal activity for neuroscience research. Due to the nonlinear two-photon absorption, the optical sectioning ability is inherent, resulting in two-photon images with high signal contrast and signal-to-noise
Fan, Feng +3 more
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Fluorescence lifetime-resolved imaging
Photosynthesis Research, 2009This is a short account of fluorescence lifetime-resolved imaging, in order to acquaint readers who are not experts with the basic methods for measuring lifetime-resolved signals throughout an image. We present the early FLI (fluorescence lifetime imaging) history, review shortly the instrumentation and experimental design, discuss briefly the ...
Yi-Chun, Chen, Robert M, Clegg
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Fluorescence image-guided neurosurgery
Future Oncology, 2017Surgery plays an important role in the management of high-grade gliomas (HGG) and imparts significant tumor-free and overall survival advantages. However HGG margins are often invisible, making their gross total resection (GTR) a difficult task. Hence intraoperative technology such as intraoperative fluorescence was a revolutionary discovery.
Sadao, Kaneko, Muftah S, Eljamel
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Live‐Cell Fluorescence Imaging
2007This chapter examines the ways to optimize the signal-to-noise ratio while keeping the specimen healthy. Live cells expressing fluorescent protein fusions are usually dim compared to fixed specimens, both because the fluorescent proteins are not very bright and because there is, in most cases, only one fluorophores per protein.
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Spectral imaging fluorescence microscopy
Genes to Cells, 2002The spectral resolution of fluorescence microscope images in living cells is achieved by using a confocal laser scanning microscope equipped with grating optics. This capability of temporal and spectral resolution is especially useful for detecting spectral changes of a fluorescent dye; for example, those associated with fluorescence resonance energy ...
Tokuko, Haraguchi +4 more
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Fluorescence Lifetime Imaging Microscopy
2007Publisher Summary Fluorescence lifetime imaging microscopy (FLIM) produces spatially resolved images of fluorophore lifetime (the property describing how rapidly fluorescence decays), providing another dimension of information for visualizing fluorophores and an additional source of contrast.
Ching-Wei, Chang +2 more
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