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Subdiffraction-Resolution Fluorescence Imaging with Conventional Fluorescent Probes†
Angewandte Chemie - International Edition, 2008Mark Schüttpelz +2 more
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Chlorophyll Fluorescence Imaging
2018Chlorophyll fluorescence imaging provides a noninvasive rapid screen to assess the physiological status of a number of leaves or plants simultaneously. Although there are no standard protocols for chlorophyll fluorescence imaging, here we provide an example of routines for some of the typical measurements.
Lawson, Tracy, Vialet-Chabrand, Silvere
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Digital imaging fluorescence microscopy
1986For digital imaging fluorescence microscopy we have used a Grinnell 274 image processor interfaced to a Lab Datex LSI 11/23 under RT-11 controlled by Integrated Solutions 68010 operating under Unix BSD 4.2. Experimental approaches to address (a) photobleaching of the fluorophore, (b) misalignment of images by nonparfocal microscope optics and (c) the ...
Smith, L.C. +3 more
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FLUORESCENCE MOLECULAR IMAGING
Annual Review of Biomedical Engineering, 2006There is a wealth of new fluorescent reporter technologies for tagging of many cellular and subcellular processes in vivo. This imposed contrast is now captured with an increasing number of available imaging methods that offer new ways to visualize and quantify fluorescent markers distributed in tissues.
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Multimodal Fluorescence Imaging Spectroscopy
2013Multimodal fluorescence imaging is a versatile method that has a wide application range from biological studies to materials science. Typical observables in multimodal fluorescence imaging are intensity, lifetime, excitation, and emission spectra which are recorded at chosen locations at the sample.
Stopel, M.H. +3 more
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Fluorescence lifetime imaging of coral fluorescent proteins
Microscopy Research and Technique, 2007AbstractCorals, like many other coelenterates, contain fluorescent pigments that show considerable homology with the well known green fluorescent protein of the jellyfish Aequoria. In corals, unlike jellyfish, multiple proteins are present and the range of excitations and emissions suggest the possibility of energy transfer.
Guy, Cox, Mikhail, Matz, Anya, Salih
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Live‐Cell Fluorescence Imaging
2007This chapter examines the ways to optimize the signal-to-noise ratio while keeping the specimen healthy. Live cells expressing fluorescent protein fusions are usually dim compared to fixed specimens, both because the fluorescent proteins are not very bright and because there is, in most cases, only one fluorophores per protein.
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Fluorescence Lifetime Imaging Microscopy
Current Protocols in Cell Biology, 2004AbstractFluorescent lifetime imaging microscopy is a powerful tool to enhance the contrast in images of biological samples and to investigate the local environment of a fluorochrome. FLIM allows the detection of protein‐protein interactions and their biochemical state by the quantitative detection of Förster resonance energy transfer (FRET) between ...
Alessandro, Esposito, Fred S, Wouters
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Spectral imaging fluorescence microscopy
Genes to Cells, 2002The spectral resolution of fluorescence microscope images in living cells is achieved by using a confocal laser scanning microscope equipped with grating optics. This capability of temporal and spectral resolution is especially useful for detecting spectral changes of a fluorescent dye; for example, those associated with fluorescence resonance energy ...
Tokuko, Haraguchi +4 more
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Image analysis of catecholamine fluorescence
Brain Research Bulletin, 1982Image analysis methods are being developed for measuring the density and distribution of perivascular noradrenergic nerves and their varicosities, demonstrated by fluorescence histochemistry. Image analysis is recommended in comparison with other methods for the quantitation of fluorescence, because image resolution is greater and larger areas of ...
T, Cowen, G, Burnstock
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