Results 181 to 190 of about 2,637,757 (313)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Ninth Flyer 11_1_2001

open access: yes, 2014
The quarterly newsletter, The Ninth Flyer, published by the Ninth Air Force Association, Inc.
9th Air Force Association
core  

Characterization and Optimization of Intelligent Dampers Based on Bionic Principles. [PDF]

open access: yesBiomimetics (Basel)
Guo N   +6 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Ninth Flyer Winter_1995

open access: yes, 2014
The quarterly newsletter, The Ninth Flyer, published by the Ninth Air Force Association, Inc.
9th Air Force Association
core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Ninth Flyer 3_4_1993

open access: yes, 2014
The quarterly newsletter, The Ninth Flyer, published by the Ninth Air Force Association, Inc.
9th Air Force Association
core  

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

open access: yesFEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke   +4 more
wiley   +1 more source

Ninth Flyer 16_4_2006

open access: yes, 2014
The quarterly newsletter, The Ninth Flyer, published by the Ninth Air Force Association, Inc.
9th Air Force Association
core  

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