Results 1 to 10 of about 156,558 (310)

Ensemble Force Spectroscopy by Shear Forces. [PDF]

J Vis Exp, 2022
Single-molecule techniques based on fluorescence and mechanochemical principles provide superior sensitivity in biological sensing. However, due to the lack of high throughput capabilities, the application of these techniques is limited in biophysics. Ensemble force spectroscopy (EFS) has demonstrated high throughput in the investigation of a massive ...
Pokhrel P, Hu C, Mao H.
europepmc   +3 more sources

Protein structure prediction in the era of AI: Challenges and limitations when applying to in silico force spectroscopy [PDF]

Frontiers in Bioinformatics, 2022
Mechanoactive proteins are essential for a myriad of physiological and pathological processes. Guided by the advances in single-molecule force spectroscopy (SMFS), we have reached a molecular-level understanding of how mechanoactive proteins sense and ...
Priscila S. F. C. Gomes, Diego E. B. Gomes, Rafael C. Bernardi   +2 more
doaj   +2 more sources

Force spectroscopy with electromagnetic tweezers. [PDF]

J Appl Phys, 2021
Force spectroscopy using magnetic tweezers (MTs) is a powerful method to probe the physical characteristics of single polymers. Typically, molecules are functionalized for specific attachment to a glass surface at one end and a micrometer-scale paramagnetic bead at the other end.
Piccolo JG, Méndez Harper J, McCalla D, Xu W, Miller S, Doan J, Kovari D, Dunlap D, Finzi L.   +8 more
europepmc   +5 more sources

Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature [PDF]

Sensors, 2021
Atomic force microscopy is an extremely versatile technique, featuring atomic-scale imaging resolution, and also offering the possibility to probe interaction forces down to few pN.
Reinier Oropesa-Nuñez, Andrea Mescola, Massimo Vassalli, Claudio Canale   +3 more
doaj   +2 more sources

Near equilibrium unbinding of streptavidin/biotin using single molecule acoustic force spectroscopy [PDF]

Saravanan Y, Villanueva L, Leveque C, Modesti M, Far OE, Valotteau C, Rico F.   +6 more
europepmc   +2 more sources

Force spectroscopy reveals membrane fluctuations and surface adhesion of extracellular nanovesicles impact their elastic behavior. [PDF]

Proc Natl Acad Sci U S A
Stridfeldt F, Pandey V, Kylhammar H, Talebian Gevari M, Metem P, Agrawal V, Görgens A, Mamand DR, Gilbert J, Palmgren L, Holme MN, Gustafsson O, El Andaloussi S, Mitra D, Dev A.   +14 more
europepmc   +3 more sources

Current and future perspectives of atomic force microscopy to elicit the intrinsic properties of soft matter at the single molecule level

AIMS Bioengineering, 2022
Soft matter encompasses multitude of systems like biomolecules, living cells, polymers, composites or blends. The increasing interest to better understand their physico-chemical properties has significantly favored the development of new techniques with ...
Carlos Marcuello
doaj   +1 more source

Acoustic force spectroscopy [PDF]

Nature Methods, 2014
Force spectroscopy has become an indispensable tool to unravel the structural and mechanochemical properties of biomolecules. Here we extend the force spectroscopy toolbox with an acoustic manipulation device that can exert forces from subpiconewtons to hundreds of piconewtons on thousands of biomolecules in parallel, with submillisecond response time ...
Gerrit Sitters, Douwe Kamsma, Gregor Thalhammer, Monika Ritsch-Marte, Erwin J G Peterman, Gijs J L Wuite   +5 more
openaire   +3 more sources

Statistical Study of Low-Intensity Single-Molecule Recognition Events Using DeepTip^TM Probes: Application to the Pru p 3-Phytosphingosine System

Biomimetics, 2023
The interaction between the plant lipid transfer protein Pru p 3 and phytosphingosine was assessed using an atomic force microscope. Phytosphingosine was covalently immobilized on DeepTipTM probes and Pru p 3 on MicroDeckTM functionalized substrates ...
Rafael Daza, María Garrido-Arandia, Daniel Corregidor-Ortiz, Carla Isabel Pérez, Luis Colchero, Raquel Tabraue-Rubio, Manuel Elices, Gustavo V. Guinea, Araceli Diaz-Perales, José Pérez-Rigueiro   +9 more
doaj   +1 more source

Elucidating the Influence of Lipid Composition on Bilayer Perturbations Induced by the N-Terminal Region of the Huntingtin Protein

Biophysica, 2023
Understanding the membrane interactions of the N-terminal 17 residues of the huntingtin protein (HttN) is essential for unraveling its role in cellular processes and its impact on huntingtin misfolding. In this study, we used atomic force microscopy (AFM)
Yasith Indigahawela Gamage, Jianjun Pan
doaj   +1 more source