Results 1 to 10 of about 529,778 (173)
Ensemble Force Spectroscopy by Shear Forces. [PDF]
J Vis Exp, 2022 Single-molecule techniques based on fluorescence and mechanochemical principles provide superior sensitivity in biological sensing. However, due to the lack of high throughput capabilities, the application of these techniques is limited in biophysics. Ensemble force spectroscopy (EFS) has demonstrated high throughput in the investigation of a massive ...
Pokhrel P, Hu C, Mao H.
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Protein structure prediction in the era of AI: Challenges and limitations when applying to in silico force spectroscopy [PDF]
Frontiers in Bioinformatics, 2022 Mechanoactive proteins are essential for a myriad of physiological and pathological processes. Guided by the advances in single-molecule force spectroscopy (SMFS), we have reached a molecular-level understanding of how mechanoactive proteins sense and ...
Priscila S. F. C. Gomes +2 more
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Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature [PDF]
Sensors, 2021 Atomic force microscopy is an extremely versatile technique, featuring atomic-scale imaging resolution, and also offering the possibility to probe interaction forces down to few pN.
Reinier Oropesa-Nuñez +3 more
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Force spectroscopy with electromagnetic tweezers. [PDF]
J Appl Phys, 2021 Force spectroscopy using magnetic tweezers (MTs) is a powerful method to probe the physical characteristics of single polymers. Typically, molecules are functionalized for specific attachment to a glass surface at one end and a micrometer-scale paramagnetic bead at the other end.
Piccolo JG +8 more
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Emerging Microbes and Infections, 2022 The binding of the receptor binding domain (RBD) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein onto human angiotensin-converting enzyme 2 (ACE2) is considered as the first step for the virus to adhere onto the host cells ...
Xiaoxu Zhang +10 more
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Acoustic force spectroscopy [PDF]
Nature Methods, 2014 Force spectroscopy has become an indispensable tool to unravel the structural and mechanochemical properties of biomolecules. Here we extend the force spectroscopy toolbox with an acoustic manipulation device that can exert forces from subpiconewtons to hundreds of piconewtons on thousands of biomolecules in parallel, with submillisecond response time ...
Gerrit Sitters +5 more
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Nanomaterials, 2022 The environmental oxygen level plays a critical role in corneal crosslinking (CXL), a treatment method to increase corneal biomechanical stability. In this study, we introduce a new CXL method (Bubble-CXL), in which intracameral oxygen serves as an ...
Ammar Alkhalde +5 more
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Biophysica, 2023 Understanding the membrane interactions of the N-terminal 17 residues of the huntingtin protein (HttN) is essential for unraveling its role in cellular processes and its impact on huntingtin misfolding. In this study, we used atomic force microscopy (AFM)
Yasith Indigahawela Gamage, Jianjun Pan
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Single-cell force spectroscopy [PDF]
Journal of Cell Science, 2008 The controlled adhesion of cells to each other and to the extracellular matrix is crucial for tissue development and maintenance. Numerous assays have been developed to quantify cell adhesion. Among these, the use of atomic force microscopy (AFM) for single-cell force spectroscopy (SCFS) has recently been established. This assay permits the adhesion of
Helenius, J. +3 more
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Concurrent Atomic Force Spectroscopy [PDF]
Communications Physics, 2018 ABSTRACTForce-spectroscopy by Atomic Force Microscopy (AFM) is the technique of choice to measure mechanical properties of molecules, cells, tissues and materials at the nano and micro scales. However, unavoidable calibration errors of AFM probes make it cumbersome to quantify modulation of mechanics.
Carolina Pimenta-Lopes +4 more
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