Results 131 to 140 of about 10,729,116 (245)

Finite element method for fractional order parabolic obstacle problem with nonlinear source term

Partial Differential Equations in Applied Mathematics
This paper considers the finite element method for a fractional order parabolic obstacle problem in time with a nonlinear source term. An error estimation of the spatially semi-discrete problem is discussed.
Allaoua Mehri, Hakima Bouhadjera, Mohammed S. Abdo, Hadeel Z. Alzumi, Wafa Shammakh   +4 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Fractional Order Systems:PID Controller Design and Fractional Order Element Modeling

, 2014
This research thesis within the XXVI Ph.D. Course in Systems Engineering is centered on Fractional, or also noticed as Non-Integer Order, Systems. The Ph.D. Thesis' purpose is to present the auto-tuning procedure of Fractional Order PID Controllers PID proposing also a future implementation via fractional order elements. The Thesis is organized in four
openaire   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

A Galerkin finite element technique with Iweobodo-Mamadu-Njoseh wavelet (IMNW) basis function for the solution of time-fractional advection–diffusion problems

Partial Differential Equations in Applied Mathematics
In this paper, the authors used wavelet-based Galerkin finite element technique constructed with Iweobodo-Mamadu-Njoseh wavelet as the basis function, for the numerical solution of time-fractional advection–diffusion equations.
D.C. Iweobodo, G.C. Abanum, N.I. Ochonogor, J.S. Apanapudor, I.N. Njoseh   +4 more
doaj   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Implementation Method and Bench Testing of Fractional-Order Biquadratic Transfer Function-Based Mechatronic ISD Suspension

Sensors
To address the challenge of physically realizing fractional-order electrical networks, this study proposes an implementation method for a mechatronic inerter–spring–damper (ISD) suspension based on a fractional-order biquadratic transfer function ...
Yujie Shen, Dongdong Qiu, Haolun Xu, Yanling Liu, Kecheng Sun, Xiaofeng Yang, Yan Guo   +6 more
doaj   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source