Results 81 to 90 of about 15,676,085 (372)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

open access: yesFEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho   +3 more
wiley   +1 more source

The Financial Accelerator in a Quantitative Business Cycle Framework

open access: yes, 1998
This paper develops a dynamic general equilibrium model that is intended to help clarify the role of credit market frictions in business fluctuations, from both a qualitative and a quantitative standpoint.
B. Bernanke, M. Gertler, Simon Gilchrist
semanticscholar   +1 more source

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

open access: yesFEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots

open access: yesFEBS Letters, EarlyView.
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino   +7 more
wiley   +1 more source

Validation of the theoretical domains framework for use in behaviour change and implementation research

open access: yesImplementation Science, 2012
BackgroundAn integrative theoretical framework, developed for cross-disciplinary implementation and other behaviour change research, has been applied across a wide range of clinical situations.
J. Cane, Denise A. O’Connor, S. Michie
semanticscholar   +1 more source

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

open access: yesFEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue   +6 more
wiley   +1 more source

A statutory compensation model for AI training based on the copy levies payment framework [PDF]

open access: yesAnnales Scientia Politica
The article addresses the lack of adequate legal mechanisms regulating the use of copyrighted works in the training of artificial intelligence (AI) algorithms, particularly in the context of copyright law.
Andrzej Madera
doaj  

Managing Technological Knowledge within the Organisation

open access: yesSouth African Journal of Business Management, 1975
Baie Suid-Afrikaanse maatskappye wat gesofistiseerde vervaardigings­tegnologieë gebruik, koop die kennis van oorsee in die vorm van ontwerpe, instruksies, opleiding en hulp in probleemsituasies.
A. T. Morkel
doaj   +1 more source

New framework for adaptive and agile honeypots

open access: yesETRI Journal, 2020
This paper proposes a new framework for the development and deployment of honeypots for evolving malware threats. As new technological concepts appear and evolve, attack surfaces are exploited.
Seamus Dowling   +2 more
doaj   +1 more source

A guide to using the Theoretical Domains Framework of behaviour change to investigate implementation problems

open access: yesImplementation Science, 2017
BackgroundImplementing new practices requires changes in the behaviour of relevant actors, and this is facilitated by understanding of the determinants of current and desired behaviours.
L. Atkins   +11 more
semanticscholar   +1 more source

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