Results 261 to 270 of about 60,935 (313)

3D FRAP movies

2023
P1.23-27-34-40-44: primary cortical neurons with a wild type Mecp2 allele endogenously tagged with a HaloTag. P1.25-26-28-29-30-31-32-22-38-39: primary cortical neurons with a G118E mutant Mecp2 allele endogenously tagged with a HaloTag. P1.99: primary cortical neurons infected with a lentiviral vector expressing Halo-tagged H2B.
Claudia Cattoglio, Carlo Vetralla
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Analysis of receptor oligomerization by FRAP microscopy

Nature Methods, 2009
Here we describe an approach to investigate di- or oligomerization of transmembrane receptors in living cells with fluorescence recovery after photobleaching (FRAP). We immobilized a defined fraction of receptors with antibodies and then measured lateral mobility of the nonimmobilized fraction by FRAP.
Dorsch, Sandra, Klotz, Karl-Norbert, Engelhardt, Stefan, Lohse, Martin J, Bünemann, Moritz   +4 more
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FRAP analysis of binding: proper and fitting

Trends in Cell Biology, 2005
Dynamic molecular interactions are fundamental to all cellular processes. In vivo analyses of these interactions are frequently done using fluorescence recovery after photobleaching (FRAP). Proper interpretation of FRAP data yields information about the binding interactions of fluorescently tagged molecules, including the number of binding states and ...
Brian L, Sprague, James G, McNally
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Switching Frontal Polymerization Mechanisms: FROMP and FRaP

ACS Macro Letters, 2022
Two frontal polymerization (FP) mechanisms, frontal ring-opening metathesis polymerization (FROMP) of dicyclopentadiene and frontal radical polymerization (FRaP) of benzyl acrylate and hexanediol diacrylate, were combined for rapid manufacturing of welded thermoset materials.
Jacob J. Lessard, Parmeet Kaur, Justine E. Paul, Kelly M. Chang, Nancy R. Sottos, Jeffrey S. Moore   +5 more
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Expanding the scope of quantitative FRAP analysis

Journal of Theoretical Biology, 2010
In this study, new mathematical models were developed for analysis of fluorescence recovery after photobleaching (FRAP) data to account for features not represented in previous analysis: conical photobleaching geometry, spatial variations in binding of fluorescent molecules, and directed transport of fluorescent molecules. To facilitate computations in
Mark A, Hallen, Anita T, Layton
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Nucleocytoplasmic shuttling revealed by FRAP and FLIP technologies

Current Opinion in Biotechnology, 2005
Protein mobility within cells is of key importance for many cellular functions. Although immunostaining can reveal protein locations in the steady-state, this might not represent the full picture and provides no information about protein movements. Fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP) are two ...
Mario, Köster, Thomas, Frahm, Hansjörg, Hauser   +2 more
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Analysis of Biomolecular Dynamics by FRAP and Computer Simulation

2014
We present a Monte Carlo simulation environment for modelling complex biological molecular interaction networks and for the design, validation, and quantitative analysis of FRAP assays to study these. The program is straightforward in its implementation and can be instructed through an intuitive script language.
Geverts, Bart, Van Royen, Martin E., Houtsmuller, Adriaan B.   +2 more
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Molecular diffusion and binding analyzed with FRAP

Protoplasma, 2014
Intracellular molecular transport and localization are crucial for cells (plant cells as much as mammalian cells) to proliferate and to adapt to diverse environmental conditions. Here, some aspects of the microscopy-based method of fluorescence recovery after photobleaching (FRAP) are introduced.
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Once a physicist: Thomas Fraps

Physics World, 2006
My interest in science was first sparked by my grandfather, who was an engineer and mathematician. Later I started reading science-fiction and popular-science books by the likes of Carl Sagan and Douglas Hofstadter. I finally decided to study physics when I was doing my military service, which would have been an intellectual vacuum for me had it not ...
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FRAP Analysis of Extracellular Diffusion in Zebrafish Embryos

2018
Morphogens are signaling molecules that provide positional information to cells during development. They must move through embryonic tissues in order to coordinate patterning. The rate of a morphogen's movement through a tissue-its effective diffusivity-affects the morphogen's distribution and therefore influences patterning.
Soh, Gary H., Müller, Patrick
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