Results 91 to 100 of about 1,562,611 (347)

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

open access: yesFEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel   +4 more
wiley   +1 more source

"Congress shall make no law abridging the freedom of speech" posters [PDF]

open access: yes, 2017
These posters were created by the Mugar Greene Scholars to promote free ...
Richards, Jessica
core  

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

The Value of Dissent [PDF]

open access: yes, 2000
This essay reviews Dissent, Injustice, and the Meanings of America by Steven H. Shiffrin (1999). Theorizing about the freedom of speech has been a central enterprise of contemporary legal scholarship.
Solum, Lawrence B.
core   +1 more source

“When My Own Writing is My Enemy”: Notes on Perumal Murugan and an Interview

open access: yesSouth Asia Multidisciplinary Academic Journal, 2023
Laetitia Zecchini
doaj   +1 more source

Are There Limits to Free Speech?

open access: yesتأملات اخلاقی, 2021
Freedom of speech has traditionally been a cause championed by the left and liberal side of the political spectrum, against conservatives who have tried to limit the expression of radical ideas.
Peter Singer
doaj  

A liberdade de expressão, o direito ao esquecimento e a proteção da intimidade: uma análise jurídica dos conflitos na era digital

open access: yesRevista Interdisciplinar de Direito, 2019
This article will deal with the correlation between some of the fundamental rights found in the constitutional order and the digital tools used to exchange and send information.
Diogo Oliveira Muniz Caldas
doaj   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

open access: yesFEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Resolving content moderation dilemmas between free speech and harmful misinformation. [PDF]

open access: yesProc Natl Acad Sci U S A, 2023
Kozyreva A   +6 more
europepmc   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

open access: yesFEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee   +6 more
wiley   +1 more source

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