Results 111 to 120 of about 20,788 (171)
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Bidirectional shadowing in freeze‐etching
Journal of Microscopy, 1983SUMMARYBidirectional shadowing in freeze‐etching may be achieved by firing an electron‐beam shadowing source, rotating the specimen stage through a desired angle, and re‐firing the shadowing source. It is demonstrated that portrait shadow‐casting, which permits information to be drawn from much of the specimen region lying within primary shadows, can ...
J H, Willison, R D, Moir
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1972
Publisher Summary The first electron micrographs of bacterial cells were published about 20 years ago. These early studies suggested the presence of definite intracellular structures. Sectioning techniques, originally developed for eucaryotic cells, were gradually modified so that thin sections of bacteria could be obtained. These first thin sections
C C, Remsen, S W, Watson
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Publisher Summary The first electron micrographs of bacterial cells were published about 20 years ago. These early studies suggested the presence of definite intracellular structures. Sectioning techniques, originally developed for eucaryotic cells, were gradually modified so that thin sections of bacteria could be obtained. These first thin sections
C C, Remsen, S W, Watson
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Rapid Freezing of Freeze-Etch Specimens
Proceedings, annual meeting, Electron Microscopy Society of America, 1980The importance of a high rate of temperature decrease (“rapid freezing”) when freezing specimens for freeze-etching has long been recognized1. The two basic methods for achieving rapid freezing are: 1) dropping the specimen onto a metal surface at low ...
A. Elgsaeter, T. Espevik, G. Kopstad
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Journal of Microscopy, 1978
SUMMARYA new freeze‐etch unit is described in which one can make replicas where hydrated, frozen, fractured specimens rotate during specimen heavy metal shadowing. The freeze‐etch unit can also be used to do low temperature freeze‐fracturing and conventional freeze‐etching.
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SUMMARYA new freeze‐etch unit is described in which one can make replicas where hydrated, frozen, fractured specimens rotate during specimen heavy metal shadowing. The freeze‐etch unit can also be used to do low temperature freeze‐fracturing and conventional freeze‐etching.
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Complementary freeze-fracture and freeze-etch preparations
Proceedings, annual meeting, Electron Microscopy Society of America, 1984Whereas freeze-etching technology was introduced in 1957 (1) and the first freeze-etching equipment to be made available commercially was developed in 1961 (2), the need for complementary replicas was not recognized until Branton (3) proposed that the two membrane faces seen in freeze-etched specimens were not the inner and outer surfaces of membranes ...
Russell L. Steere +2 more
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Visualization of ribosomes by freeze-etching
Experimental Cell Research, 1970Abstract Ribosomes are revealed on the outer surface of the nucler envelope by etching freeze-fractured nuclei isolated from sea urchin embryos. Etching techniques provide a novel way of investigating ribosome-membrane association.
J, Wartiovaara, D, Branton
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Freeze‐etch observations of rat lung
The Anatomical Record, 1971AbstractThe freeze‐etch technique was used to study the fine structure of the terminal respiratory membranes of rat lung. Special emphasis was given to the ultrastructure of the type II alveolar cell and its role in formation of the surface active material.
J C, Belton +3 more
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Freeze-etching studies on muscle
Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 1971The technique of freeze-etching is illustrated with reference to striated muscle. Besides features of immediate biological interest, the material demonstrates various ways in which the process may be used in general to yield new information.
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Freeze-etching observations of trichophyton violaceum
Medical Mycology, 1977Trichophyton violaceum was cultured on a Sabouraud's dextrose agar slants for freeze-fracture replication. It was cryoprotected with glycerol and sucrose. The surface structure of the cell wall showed a rodlet pattern. Invagination-like craters were observed in the plasmalemma.
T, Hasegawa, E, Nakai, V S, Rajan
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Freeze‐etching and dental research
Journal of Periodontal Research, 1972Dental plaque is the aetiologie factor common to caries and chronic periodontitis. Understanding of the structure of this microbial film has been hindered by the fact that conventional methods of fixation and embedding are more or less disruptive in their effects.
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