Results 121 to 130 of about 20,788 (171)

Freeze-etched surfaces in potato starch

Journal of Ultrastructure Research, 1972
Native and Lintnerized potato starch grains were freeze-fractured and freezeetched. All samples showed a granular surface. The Lintnerized samples, in addition, presented unique structures which have been described as microfibrils. The question, whether the granules and the microfibrils seen here are real structures in the starch grain or artifacts ...
R, Leonard, C, Sterling
openaire   +2 more sources

Freeze-etching and direct observation of freezing damage

Cryobiology, 1969
Summary Freeze-etching, as a method of preparing specimens for electron microscopy, is perhaps the best method for visualizing the fine structure of freezing damage to cells. New equipment permits the preparation of multiple specimens and double replicas, and rapid temperature changes.
openaire   +2 more sources

Freeze-Etching in Neuroanatomy

1981
The application of the freeze-etching technique as described by Moor and Muhlethaler (1963) to neural tissue was introduced by Bischoff (Bischoff and Moor 1967 a, b) for the study of myelin and by Akert et al. (1969) in an attempt to visualize the active sites of the presynaptic membrane.
K. Akert, C. Sandri, H. Moor
openaire   +1 more source

Interpreting the results of freeze‐etching

Journal of Microscopy, 1977
SUMMARYMorphological data obtained by freeze‐fracturing and other low temperature techniques must be interpreted in terms of molecular organization and function. Interpretation is aided by physical and biochemical approaches. Physical approaches such as rotary replication and ultralow temperature fracturing can improve resolution and preserve molecular
D, Branton, S, Kirchanski
openaire   +2 more sources

Preparation of Coenocytes for Freeze-Etching

Stain Technology, 1975
Successful freeze-etching of a coenocyte has been accomplished with glutaraldehyde stabilization followed by infiltration with cryoprotectant. Hyphae of the coenocytic water mold Achlya were stabilized with 5% glutaraldehyde in phosphate buffer.
E A, Ellis, J T, Mullins
openaire   +2 more sources

The freezing rate of freeze-etch specimens for electron microscopy

Cryobiology, 1974
Abstract Experiments on the rapid freezing of freeze-etch sized specimens have shown this rate between 0 and −100 °C to be approximately 1000 °C/sec. This is much faster than the rate of 100 °C/sec estimated by Moor for specimens cooled in liquid Freon 12. Heat transfer from the rapidly immersed specimen and mount appears to be mainly through forced
A J, Glover, Z S, Garvitch
openaire   +2 more sources

Freeze-Etching and Freeze-Fracturing

1973
The freeze-etching (or fracturing) technique involves the making of a platinum-carbon replica of the fracture face through frozen cells. This replica is examined in the electron microscope. There are two advantages compared with earlier techniques. Firstly, fixation and dehydration are not necessary and the replica is thus of cells which differ from ...
openaire   +1 more source

Freeze‐fracturing and freeze‐etching of cardiac myosin filaments

Journal of Microscopy, 1975
SUMMARYMyofilament structure was studied in freeze‐etch replicas of unfixed, glycerinated beef cardiac muscle. The information which is revealed depends upon the direction of metal shadowing in relation to the filament axis. Shadows oblique to this axis reveal that the outer surface of a longitudinal half of a thick filament comprises three, sometimes ...
openaire   +2 more sources

Stereography of Freeze Etched Replicas

Proceedings, annual meeting, Electron Microscopy Society of America, 1969
The frequent analogy made between the appearance of freeze etch replicas and the lunar landscape is appropriate in the sense that both are best viewed as if they were opaque irregular surfaces illuminated from behind and to the side of the observer. Replicas are actually illuminated by a transmitted electron beam and the illusion of a lighted opaque ...
James H. McAlear, Marie Shatos, Anthony Demsey, Anita Donovan   +3 more
openaire   +1 more source

New Dimensions in Freeze-Etching

Proceedings, annual meeting, Electron Microscopy Society of America, 1969
A redesigned specimen holder and cap have made possible the freeze-etching of both fracture surfaces of a frozen fractured specimen. In principal, the procedure involves freezing a specimen between two specimen holders (as shown in A, Fig. 1, and the left side of Fig. 2).
Russell L. Steere, Michael Moseley
openaire   +1 more source