Results 171 to 180 of about 5,792 (234)
Some of the next articles are maybe not open access.
Recent progress in the freeze-etching technique
Philosophical Transactions of the Royal Society of London Series B, Biological Sciences, 1971Abstract The freeze-etching technique must be improved if structures at the molecular size level are to be seen. The limitations of the technique are discussed here together with the progress made in alleviating them. The vitrification of living specimens is limited by the fact that very high freezing rates are needed.
H Moor
exaly +3 more sources
Freeze Fracture and Freeze Etching
2013Freeze fracture depends on the property of frozen tissues or cells, when cracked open, to split along the hydrophobic interior of membranes, thus revealing broad panoramas of membrane interior. These large panoramas reveal the three-dimensional contours of membranes making the methods well suited to studying changes in membrane architecture.
Douglas E, Chandler, William P, Sharp
openaire +2 more sources
Complementary freeze‐fracture, freeze‐etch specimens
Journal of Microscopy, 1979SUMMARYA procedure is described for the preparation and comparison of complementary freeze‐fracture‐freeze‐etch specimens. These complementary replicas reveal the value of etching some specimens even when the cryoprotectant concentration amounts to as much as 25% glycerol and 25% sucrose.
R L, Steere, E F, Erbe
openaire +2 more sources
1972
Publisher Summary The first electron micrographs of bacterial cells were published about 20 years ago. These early studies suggested the presence of definite intracellular structures. Sectioning techniques, originally developed for eucaryotic cells, were gradually modified so that thin sections of bacteria could be obtained. These first thin sections
C C, Remsen, S W, Watson
openaire +2 more sources
Publisher Summary The first electron micrographs of bacterial cells were published about 20 years ago. These early studies suggested the presence of definite intracellular structures. Sectioning techniques, originally developed for eucaryotic cells, were gradually modified so that thin sections of bacteria could be obtained. These first thin sections
C C, Remsen, S W, Watson
openaire +2 more sources
Bidirectional shadowing in freeze‐etching
Journal of Microscopy, 1983SUMMARYBidirectional shadowing in freeze‐etching may be achieved by firing an electron‐beam shadowing source, rotating the specimen stage through a desired angle, and re‐firing the shadowing source. It is demonstrated that portrait shadow‐casting, which permits information to be drawn from much of the specimen region lying within primary shadows, can ...
J H, Willison, R D, Moir
openaire +2 more sources
Preparation of Coenocytes for Freeze-Etching
Stain Technology, 1975Successful freeze-etching of a coenocyte has been accomplished with glutaraldehyde stabilization followed by infiltration with cryoprotectant. Hyphae of the coenocytic water mold Achlya were stabilized with 5% glutaraldehyde in phosphate buffer.
E A, Ellis, J T, Mullins
openaire +2 more sources
Proceedings, annual meeting, Electron Microscopy Society of America, 1968
Freeze-etching, as a tool in the preparation of biological specimens for electron microscopy, has seen relatively little use since it was introduced in 1957. The involved procedure, originally described, and, until recently, the cost of special equipment, must certainly have played a part in the failure of freeze-etching to become a commonly used ...
openaire +2 more sources
Freeze-etching, as a tool in the preparation of biological specimens for electron microscopy, has seen relatively little use since it was introduced in 1957. The involved procedure, originally described, and, until recently, the cost of special equipment, must certainly have played a part in the failure of freeze-etching to become a commonly used ...
openaire +2 more sources