Results 181 to 190 of about 4,894,783 (245)

Development of a highly efficient prime editing platform for cucurbits enables breeding of multi-disease-resistant cucumber. [PDF]

J Integr Plant Biol
Wang J, Xiao L, Xin T, Li J, Yang X.
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Establishment of an Agrobacterium-Mediated Genetic Transformation System for Prunus sibirica and Its Preliminary Application in PsWRKY17 Overexpression Under Low-Temperature Stress. [PDF]

Physiol Plant
Wang S, Chen J, Sun Y, Liu R, Liu Q, Dong S.   +5 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Genetic and functional characterization of the natural transformation system in <i>Streptococcus constellatus</i>. [PDF]

Microbiology (Reading)
Sagen AS, Shawrob KSM, Salvadori G, Junges R.   +3 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

How Hilti masters transformation

, 2011
Schmiedel, Theresa, Petry, Martin, vom Brocke, Jan   +2 more
core   +2 more sources

Comprehensive High-Sensitivity Mutation Profiling in MPNs: Diagnostic and Prognostic Implications. [PDF]

Cancers (Basel)
Kim N, Kang Y, Kook HW, Chung H, Jang JE, Lee ST, Song J, Kim JS, Choi JR, Cheong JW, Shin S.   +10 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Multi-View Occluded License Plate Recognition: A Feature Fusion Method Based on Spatial Wavelet Transform. [PDF]

Sensors (Basel)
Pan N, Zhang K, Ghaffar MA, Peng L.
europepmc   +1 more source