Microbial fructosyltransferases and the role of fructans [PDF]
Microbial fructosyltransferases are polymerases that are involved in microbial fructan (levan, inulin and fructo-oligosaccharide) biosynthesis. Structurally, microbial fructosyltransferase proteins share the catalytic domain of glycoside hydrolases 68 family and are grouped in seven phylogenetically related clusters. Fructosyltransferase-encoding genes
M L, Velázquez-Hernández +5 more
openaire +2 more sources
Streptococcus mutansfructosyltransferase interactions with glucans [PDF]
Streptococcus mutans utilizes sucrose to synthesize glucans by glucosyltransferase and fructans by fructosyltransferase (FTF). Antibodies raised against a recombinant FTF were used to study S. mutans FTF secretion. Low amounts of cell-free FTF were found in culture of S.
Ramona, Rozen +2 more
openaire +2 more sources
Synthesis of fructo-oligosaccharides using grape must and sucrose as raw materials [PDF]
Grape must market has been rising and there is an increasing interest to use it as a natural replacement for traditional sugars. Food or beverages with prebiotic compounds, including fructo-oligosaccharides (FOS), emerge as an alternative for the new ...
Gomez Zavaglia, Andrea +3 more
core +1 more source
Genetic regulation of fructosyltransferase in Streptococcus mutans [PDF]
Streptococcus mutans possesses several extracellular sucrose-metabolizing enzymes which have been implicated as important virulence factors in dental caries. This study was initiated to investigate the genetic regulation of one of these enzymes, the extracellular fructosyltransferase (Ftf).
D L, Kiska, F L, Macrina
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Synthesis of several fructo-oligosaccharides by asparagus fructosyltransferases. [PDF]
Nine fructo-oligosaccharides, synthesized in vitro from sucrose by an enzyme preparation from asparagus roots, were isolated and their structures were elucidated to be 1F (1-β-fructo-furanosyl)n sucrose [n=1 (1-kestose), 2 (nystose) and 3], 6G (1-β-fructofuranosyl)n, sucrose [n=1 (neokestose), 2 and 3] and 1F (1-β-fructofuranosyl)m 6G (1-β ...
Norio Shiomi, Jiro Yamada, Masao Izawa
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pKa modulation of the acid/base catalyst within GH32 and GH68: a role in substrate/inhibitor specificity? [PDF]
Glycoside hydrolases of families 32 (GH32) and 68 (GH68) belong to clan GH-J, containing hydrolytic enzymes (sucrose/fructans as donor substrates) and fructosyltransferases (sucrose/fructans as donor and acceptor substrates). In GH32 members, some of the
Shuguang Yuan +5 more
doaj +1 more source
Abstract Recent RNA sequencing studies have given us a deeper insight into the cariogenic impact of carbohydrate sources in the bacterium Streptococcus mutans, the principal microbial agent in dental caries etiopathogenesis. The process of dental caries development is facilitated by the ability of this bacterium to ferment some carbohydrates into ...
Veronika Jurakova +11 more
wiley +1 more source
Synthesis of a fructosyltransferase by Streptococcus sanguis [PDF]
Description pour la premiere fois d'une synthese de fructane soluble et insoluble par S. sanguis. Un rapport Na + /K + faible est necessaire pour la synthese et la secretion de la fructosyltransferase par S ...
Keevil, C. William +3 more
openaire +3 more sources
Crystallization and preliminary X-ray diffraction analysis of levansucrase (LsdA) from Gluconacetobacter diazotrophicus SRT4 [PDF]
The endophytic bacterium Gluconacetobacter diazotrophicus SRT4 secretes a constitutively expressed levansucrase (LsdA; EC 2.4.1.10), which converts sucrose to fructo-oligosaccharides and levan.
Davies, Gideon J. +7 more
core +1 more source
New insights into plant glycoside hydrolase family 32 in Agave species
In order to optimize the use of agaves for commercial applications, an understanding of fructan metabolism in these species at the molecular and genetic level is essential.
Emmanuel eAvila-de Dios +3 more
doaj +1 more source

