Results 51 to 60 of about 905 (166)

Simultaneous Knockout of Tk1‐SST and Tk1‐FFT via CRISPR/Cas9 Enhances the Natural Rubber Accumulation in Taraxacum kok‐saghyz

Plant Biotechnology Journal, Volume 23, Issue 12, Page 5654-5676, December 2025.
ABSTRACT Taraxacum kok‐saghyz (TKS) synthesises natural rubber (NR) and inulin using sucrose as a carbon source. However, molecular mechanisms regulating inulin and NR accumulation remain largely unclear. Here, we report the generation of double‐gene homozygous mutants, 1‐sst1‐fft, by simultaneously knocking out two key genes responsible for inulin ...
Bi Qin, Xue Hou, Songle Fan, Chuang Li, Xiaoxiao Wang, Qiuhui Chen, Yushuang Yang, Pin Lin, Liang RuiNan Lu, Canni Fu, Ning Yang, Qiaoli Gao, Shanshan Zheng, Shizhong Liu   +13 more
wiley   +1 more source

Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass (Lolium perenne L.)

Frontiers in Plant Science, 2016
Fructans are polymers of fructose and one of the main constituents of water-soluble carbohydrates in forage grasses and cereal crops of temperate climates.
Anna eGasperl, Annette eMorvan-Bertrand, Annette eMorvan-Bertrand, Annette eMorvan-Bertrand, Marie-Pascale ePrud'Homme, Marie-Pascale ePrud'Homme, Marie-Pascale ePrud'Homme, Eric eVan Der Graaff, Eric eVan Der Graaff, Thomas eRoitsch, Thomas eRoitsch, Thomas eRoitsch   +11 more
doaj   +1 more source

Using Natural Variation to Investigate the Function of Individual Amino Acids in the Sucrose-Binding Box of Fructan:Fructan 6G-Fructosyltransferase (6G-FFT) in Product Formation [PDF]

, 2018
Enzymes of the glycosyl hydrolase family 32 are highly similar with respect to primary sequence but catalyze divergent reactions. Previously, the importance of the conserved sucrose-binding box in determining product specificity of onion fructan:fructan ...
Ritsema, Tita, Smeekens, Sjef, Verhaar, Auke, Vijn, Irma   +3 more
core  

MEPDB: Database of microExons in plants

New Phytologist, Volume 248, Issue 1, Page 32-37, October 2025.
Summary Microexons, ≤ 51‐nucleotide (nt), particularly ultra‐short ones (1–15 nt), are challenging to identify due to their small size and frequent absence in genome annotations, which limits understanding of their biological roles. Using our developed pipeline, we identified 2398 small internal microexons across 10 diverse plant species, and most of ...
Yakub Islamov, Huihui Yu, Hongfeng Yu, Chi Zhang   +3 more
wiley   +1 more source

Evaluation of cross-linked aggregates from purified Bacillus subtilis levansucrase mutants for transfructosylation reactions

BMC Biotechnology, 2009
Background Increasing attention has been focused on inulin and levan-type oligosaccharides, including fructosyl-xylosides and other fructosides due to their nutraceutical properties.
Munguia Agustin, Rodriguez-Alegria Maria, Rudiño-Piñera Enrique, Ortiz-Soto Maria   +3 more
doaj   +1 more source

Enzyme Technology in the Food Industry: Molecular Mechanisms, Applications, and Sustainable Innovations

Food Science &Nutrition, Volume 13, Issue 9, September 2025.
This review explores the role of microbial and plant enzymes in food processing, highlighting advancements in molecular engineering and sustainable production from underutilized agro‐industrial waste. It focuses on key enzymes such as amylase, lipase, protease, and lactase, discussing their applications, advantages, and challenges in enhancing product ...
Fahima Siddikey, Md Istiakh Jahan, Hormoni, Md Toufik Hasan, Nusrat Jerin Nishi, S. M. Kamrul Hasan, Nahidur Rahman, Md. Azmain Al Faik, Mohammad Afzal Hossain   +8 more
wiley   +1 more source

Molecular Characterization of Sucrose:Sucrose 1-Fructosyltransferase and Sucrose:Fructan 6-Fructosyltransferase Associated with Fructan Accumulation in Winter Wheat during Cold Hardening [PDF]

Bioscience, Biotechnology, and Biochemistry, 2002
We isolated two cDNAs of winter wheat (Triticum aestivum L.), designated wft1 and wft2, which encoded sucrose:fructan 6-fructosyltransferase (6-SFT) and sucrose:sucrose 1-fructosyltransferase (1-SST; EC 2.4.1.99), respectively, which are involved in the synthesis of fructan in wheat.
Akira, Kawakami, Midori, Yoshida
openaire   +2 more sources

Efficient hydrolysis of raw starch and ethanol fermentation: a novel raw starch-digesting glucoamylase from Penicillium oxalicum [PDF]

, 2016
Additional file 3: Figure S2. SDS-PAGE analysis of the recombinant rPoGA15A and native PoGA15A. Lane 1, protein molecular weight marker; lane 2, the purified recombinant rPoGA15A; lane 3, the native ...
Jia-Xun Feng, Qiang-Sheng Xu, Yu-Si Yan
core   +5 more sources

Innovative Thermosensitive Composite Materials: Merging Poloxamer PF‐127 and Polysaccharides for Enhanced Functional Properties

Macromolecular Materials and Engineering, Volume 310, Issue 7, July 2025.
The poloxamer Pluronic‐F‐127‐based hydrogels are improved by the implementation of polysaccharides with different concentration ranges and structural organizations. The interactions between the polysaccharide structures and the poloxamer micellar aggregation confer the system better rheological properties and longer drug release times explained by ...
Marcos Blanco‐López, Inmaculada de Dios‐Pérez, Álvaro González‐Garcinuño, Antonio Tabernero, Eva Martín del Valle   +4 more
wiley   +1 more source

Novel biocatalysts for industrial fructooligosaccharides (FOS) production from fucosyltransferase and mutagenized invertase genes constitutively expressed in Komagataella phaffii (previously Pichia pastoris)

CyTA - Journal of Food
Fructosyltransferases (FTFs) and invertases catalyze the production of short-chain fructo-oligosaccharides (FOS), levan, and inverted syrups from sucrose—compounds that are increasingly sought after in the global food industry.
Lázaro Hernández, Carmen Menéndez, Duniesky Martínez, Luis Enrique Trujillo Toledo, Enrique Rosendo Pérez Cruz, José-Manuel Pais-Chanfrau, Ricardo Ramírez, Alina Sobrino, Gissela Estefanía Ponce Quesada, Dubiel Alfonso-González   +9 more
doaj   +1 more source