Results 81 to 90 of about 49,918 (245)

Transient Transfection of Rolling‐Circle Amplified DNA in Biomanufacturing‐Relevant Mammalian Cell Lines: A Comparison of Transfection Conditions for Optimal Protein Expression

Biotechnology and Applied Biochemistry, EarlyView.
ABSTRACT Rolling‐circle amplification (RCA) is a completely synthetic and rapid mechanism for scaling‐up bulk DNA that eliminates requirements for large‐scale bacterial fermentation. Several reports have described the surprising ability of large unprocessed (hyperbranched) rolling circle‐amplified DNA (RCA DNA) to effectively transfect cultured cells ...
Evelina Loghin, Erik Kvam, Lisa Lowery, Nathan Hudson, Jenny Keen, Brian Davis, Roger B. Dodd, Christine Surrette, John Nelson   +8 more
wiley   +1 more source

Transforming Procurement: The Dynamic Capabilities and Microfoundations to Buy Circular

Business Strategy and the Environment, EarlyView.
ABSTRACT Circular Procurement (CP) integrates Circular Economy (CE) principles into purchasing decisions to close material loops and retain value across product life cycles. Yet, its adoption remains limited due to persistent barriers within procurement processes.
Francesco Cafforio, Ilaria Giannoccaro
wiley   +1 more source

Zero Deforestation Commitments and Industry 4.0 Enabling Technologies: An Analysis of Their Role in Mitigating Deforestation

Business Strategy and the Environment, EarlyView.
ABSTRACT This study examines the role of corporate zero‐deforestation commitments (ZDCs) and Industry 4.0 (I4.0) enabling technologies in mitigating deforestation. Drawing on data from 110 companies included in the Forest 500 dataset, the research explores whether sustainability commitments and digital innovation influence firms' deforestation ...
Valentina Beretta, Eugenia Parodi, Celine Buschmann   +2 more
wiley   +1 more source

Effect of cell retention techniques in Komagataella phaffii lab‐scale continuous processes

Biotechnology Progress, EarlyView.
Abstract Perfusion technologies play a growing role in the implementation of continuous processes for biotherapeutics production in mammalian‐based manufacturing. However, their application to alternative production hosts is limited. Cell retention systems are of key importance for the efficiency of perfusion bioreactors.
Marina Y. Linova, Satish K. Kodiripaka, Edite Martins, Sobhana A. Sripada, Stefano Menegatti, John M. Woodley   +5 more
wiley   +1 more source

Evaluation of gene editing in CHO cells using the Cas‐CLOVER system

Biotechnology Progress, EarlyView.
Abstract Recent advances in gene editing technologies have transformed the genetic engineering of Chinese hamster ovary (CHO) hosts, enabling the development of cell lines with improved stability and productivity. In this study, we employed the programmable nuclease (PN) Cas‐CLOVER to precisely target the Glutamine synthetase (GS) locus in CHO cells. A
Tiffany McLamarrah, Efecan Aral, Michael Hoffman, Jennifer Tedstone, Thomas King, Jason Vitko, Maria João Sebastião, Jose M. Escandell, Mafalda M. Dias, Iona McCall, Daniel Machado, Victor Cairns, Christine DeMaria, John J. Scarcelli   +13 more
wiley   +1 more source

On the immunological evaluation of potato variety “Askol’d”

Овощи России
Relevance. Plant breeding for resistance to pests is the most radical and effective method for decreasing the enormous yield loss caused by leaf-eating, sucking, and mining insects.
N. V. Matsishina, M. V. Ermak, P. V. Fisenko, I. V. Kim   +3 more
doaj   +1 more source

Product‐Related CSR in the Digital Era: Communication Patterns That Drive Consumer Interactions on Social Media

Corporate Social Responsibility and Environmental Management, EarlyView.
ABSTRACT To meet rising sustainability demands, companies increasingly use social media to communicate product‐related CSR initiatives. Consumers' interactions with these messages largely depend on the messages' perceived credibility. However, there remains limited understanding of how firms combine communication characteristics into distinct patterns ...
Judith Derenthal, Waldemar Toporowski
wiley   +1 more source

The Squalene Oil‐in‐Water Nanoemulsion Vaccine Carrier AddaVax Potentiates TLR‐Induced B‐Cell Responses

Cytometry Part A, EarlyView.
ABSTRACT For vaccination, the mechanisms of action of antigens and of immunostimulatory pathogen‐associated molecular pattern (PAMP) adjuvants are better understood than the roles of the depot, carrier, and other formulation‐related influences. Here, we carried out cytometric studies of vaccine formulations and the effect of adjuvant formulations on B ...
Egest J. Pone, Jiin Felgner, Jenny Hernandez‐Davies, D. Huw Davies   +3 more
wiley   +1 more source

Automated CLL cell population detection using a weakly supervised approach and CLL MRD flow cytometry data

Cytometry Part B: Clinical Cytometry, EarlyView.
Abstract Minimal/measurable residual disease detection is routinely performed as part of post‐diagnostic treatment plans for many types of cancer, for which multiparameter flow cytometry is one possible modality frequently used. We propose a machine learning approach for binary prediction of minimal residual disease status with flow cytometry data. Our
Wikum Dinalankara, Chandler Sy, Jiani Chai, Paul B. Barone, Luigi Marchionni, Paul D. Simonson   +5 more
wiley   +1 more source

Straightening FSC curves

AbstractThe Fourier Shell Correlation (FSC) is traditionally calculated in resolution shells and plotted as a function of the inverse resolution. To ensure consistent statistics, these resolution shells should ideally contain an equal number of Fourier coefficients, at least approximately.
openaire   +1 more source