Results 171 to 180 of about 1,559,492 (313)

Exploring sequence-function space of a poplar glutathione transferase using designed information-rich gene variants. [PDF]

Protein Eng Des Sel, 2017
Musdal Y, Govindarajan S, Mannervik B.
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

A Hilbert cube compactification of the function space with the compact-open topology

Open Mathematics, 2009
Kogasaka Atsushi, Sakai Katsuro
doaj   +1 more source

Genomic Enzymology: Web Tools for Leveraging Protein Family Sequence-Function Space and Genome Context to Discover Novel Functions. [PDF]

Biochemistry, 2017
Gerlt JA.
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Distributed-parameter control in function space

, 1973
Tzafestas, S
core   +1 more source

Nucleation Kinetics Reveals a Distinct Biological Function Space of Biomolecular Condensates. [PDF]

Adv Sci (Weinh)
Deck LT, Pacheco SN, Ausserwöger H, Frenkel D, Erkamp NA, Knowles TPJ.   +5 more
europepmc   +1 more source

Darkness in the Human Gene and Protein Function Space: Widely Modest or Absent Illumination by the Life Science Literature and the Trend for Fewer Protein Function Discoveries Since 2000. [PDF]

Proteomics, 2018
Sinha S, Eisenhaber B, Jensen LJ, Kalbuaji B, Eisenhaber F.   +4 more
europepmc   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Exploring the sequence-function space of microbial fucosidases. [PDF]

Commun Chem
Martínez Gascueña A, Wu H, Wang R, Owen CD, Hernando PJ, Monaco S, Penner M, Xing K, Le Gall G, Gardner R, Ndeh D, Urbanowicz PA, Spencer DIR, Walsh M, Angulo J, Juge N.   +15 more
europepmc   +1 more source