This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley +1 more source
Functional Predictors of Frailty and Kidney Disease Burden in Patients with End-stage Renal Disease Receiving Dialysis. [PDF]
Ward RR +3 more
europepmc +1 more source
Convergence and Reducibility as Transferability Filters in Biomimetic Design. [PDF]
Polašek O.
europepmc +1 more source
The other giant: functional significance and divergence of Type I MADS-box genes in the evolution of land plants. [PDF]
Qiu Y, Köhler C.
europepmc +1 more source
Red Blood Cell Membrane Mechanics Using Discrete Exterior Calculus (DEC) and Optimization. [PDF]
Afas KC, Goldman D.
europepmc +1 more source
Emerging encapsulation techniques for controlled and targeted delivery of bioactive compounds in food and nutraceutical systems. [PDF]
Kumari K, Tripathy S, Srivastav PP.
europepmc +1 more source
Lignocellulosic Biomass-Based Metal-Organic Frameworks: A Sustainable Frontier for Advanced Wastewater Remediation. [PDF]
Sudarsana Babu A, Zikeli F, Puglia D.
europepmc +1 more source

