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This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu +10 more
wiley +1 more source
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The Role of Fundamental Frequency in Phonetic Accommodation
Language and Speech, 2011Previous research has argued that fundamental frequency is a critical component of phonetic accommodation. We tested this hypothesis in an auditory naming task with two conditions. Participants in an Unfiltered Condition completed an auditory naming task with a single male model talker.
Molly, Babel, Dasha, Bulatov
openaire +3 more sources
Empirical Formulation for Estimating the Fundamental Frequency of Slender Masonry Structures
Manjip Shakya +2 more
exaly +2 more sources
The Effect of Smoking on the Fundamental Frequency of the Speaking Voice
Journal of Voice, 2019OBJECTIVE Little is known about the impact of smoking on voice acoustics. Some studies have found that tobacco affects the fundamental frequency of the voice, whereas others have not.
Pauline Larrouy-Maestri +1 more
exaly +2 more sources

