Results 241 to 250 of about 1,374,086 (326)

Observation Bias in Metabarcoding

open access: yesMolecular Ecology Resources, EarlyView.
ABSTRACT DNA metabarcoding is subject to observation bias associated with PCR and sequencing, which can result in observed read proportions differing from actual species proportions in the DNA extract. Here, we amplify and sequence a mock community of known composition containing marine fishes and cetaceans using four different primer sets and a ...
Megan R. Shaffer   +5 more
wiley   +1 more source

Ancient Microbiomes as Mirrored by DNA Extracted From Century‐Old Herbarium Plants and Associated Soil

open access: yesMolecular Ecology Resources, EarlyView.
ABSTRACT Numerous specimens stored in natural history collections have been involuntarily preserved together with their associated microbiomes. We propose exploiting century‐old soils occasionally found on the roots of herbarium plants to assess the diversity of ancient soil microbial communities originally associated with these plants.
Gianluca Grasso   +7 more
wiley   +1 more source

The GEMO project: Analysis and comparison of genomes of the fungal pathogen Magnaporthe oryzae

open access: green, 2011
Cyprien Guérin   +7 more
openalex   +1 more source

Full Genome of Phialocephala scopiformis DAOMC 229536, a Fungal Endophyte of Spruce Producing the Potent Anti-Insectan Compound Rugulosin

open access: gold, 2016
Allison K. Walker   +10 more
openalex   +1 more source

A Practical Comparison of Short‐ and Long‐Read Metabarcoding Sequencing: Challenges and Solutions for Plastid Read Removal and Microbial Community Exploration of Seaweed Samples

open access: yesMolecular Ecology Resources, EarlyView.
ABSTRACT Short‐read metabarcoding analysis is the gold standard for accessing partial 16S and ITS genes with high read quality. With the advent of long‐read sequencing, the amplification of full‐length target genes is possible, but with low read accuracy.
Coralie Rousseau   +6 more
wiley   +1 more source

Needle in a Haystack: A Droplet Digital Polymerase Chain Reaction Assay to Detect Rare Helminth Parasites Infecting Natural Host Populations

open access: yesMolecular Ecology Resources, EarlyView.
ABSTRACT Helminths infect humans, livestock, and wildlife, yet remain understudied despite their significant impact on public health and agriculture. Because many of the most prevalent helminth‐borne diseases are zoonotic, understanding helminth transmission among wildlife could improve predictions and management of infection risks across species.
Chloe A. Fouilloux   +12 more
wiley   +1 more source

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