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Inserts for standard well plates are produced using vacuum forming, resulting in well‐defined bottom thicknesses down to 50 µm. After opening apertures through the bottom, hydrogels can be cast to similar thicknesses. Introducing HepG2 cells into this very thin hydrogel allows for 3D cell cultures with densities close to in vivo conditions.
Alexander Jönsson+4 more
wiley +1 more source