Results 41 to 50 of about 3,101 (257)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Fuzzy quasi-b-metric spaces

Annals of West University of Timisoara - Mathematics and Computer Science, 2022
Abstract In this paper we present a new approach for the fuzzy quasi-b-metric spaces and we obtain some properties of these spaces. A special attention is granted to the decomposition theorems of a fuzzy quasi-b-metric into a right continuous and ascending family of quasi-b-metrics.
openaire   +3 more sources

Empowering Adult Learning How AI Fuels Progress Across Disciplines and Professions

New Directions for Adult and Continuing Education, EarlyView.
ABSTRACT This article examines the evolving role of artificial intelligence (AI) as a transformative cognitive partner across educational and professional fields. It explores how AI shifts attention away from routine technical tasks and toward human‐centered skills such as critical thinking, ethical decision‐making, and professional judgment.
Rachel Wlodarsky
wiley   +1 more source

Generalization of Fixed Point Theorem for φ- Contraction Mapping of a Fuzzy b- Metric Space

Ibn Al-Haitham Journal for Pure and Applied Sciences
Let (X,W,T) be a fuzzy b- metric space, where X is a non-empty set, W is a fuzzy set on X×X×(0,∞) to [0,1], and T is a continuous t-norm, and let a function φ:[0,1]→[0,1] satisfies the following conditions: The function φ is strictly decreasing and ...
Russll Abdul Kadhim Mohammed, Zeana Zakai Jamil   +1 more
doaj   +1 more source

Some set-valued and multi-valued contraction results in fuzzy cone metric spaces

Journal of Inequalities and Applications, 2021
This paper aims to present the concept of multi-valued mappings in fuzzy cone metric spaces and prove some basic lemmas, a Hausdorff metric, and fixed point results for set-valued fuzzy cone-contraction and for multi-valued fuzzy cone-contraction ...
Saif Ur Rehman, Hassen Aydi, Gui-Xiu Chen, Shamoona Jabeen, Sami Ullah Khan   +4 more
doaj   +1 more source

On generalizations of fuzzy metric spaces

Kybernetika
Summary: The aim of the paper is to present three-variable generalizations of fuzzy metric spaces in sense of \textit{A. George} and \textit{P. Veeramani} [Fuzzy Sets Syst. 64, No. 3, 395--399 (1994; Zbl 0843.54014)] from functional and topological points of view, respectively.
Yi Shi, Wei Yao 0004
openaire   +1 more source

Vestibular Patient Journey: Insights From Vestibular Disorders Association (VeDA) Registry

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Vestibular symptoms impose a high burden of disability. Understanding real‐world diagnostic and treatment pathways can identify care gaps and guide interventions. We aimed to characterize symptom profiles, diagnostic trends, provider involvement, and treatment patterns in vestibular disorders.
Ali Rafati, Sami I. Nassar, Shaun A. Nguyen, Patricia L. Gerend, Michael C. Schubert, Michael T. Teixido, Frederick A. Godley, Joel A. Goebel, Cynthia A. Ryan, Habib G. Rizk, Amir Kheradmand   +10 more
wiley   +1 more source

Neurologic Manifestations of Long COVID Affect Adult Females More Severely Than Males

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To characterize differences in neurologic manifestations of postacute sequelae of SARS‐CoV‐2 infection (Neuro‐PASC) between females and males. Methods Cross‐sectional study of the first consecutive 261 posthospitalization Neuro‐PASC (PNP) and 2068 nonhospitalized Neuro‐PASC (NNP) patients evaluated at the Neuro‐COVID clinic between ...
Hannah Kopinsky, Melissa Lopez, Janet Miller, Millenia Jimenez, Eric M. Liotta, Igor J. Koralnik   +5 more
wiley   +1 more source