Results 31 to 40 of about 2,206,701 (242)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

COMPARATOR REGRESSION ANALYSIS IN CONDITIONS OF FUZZY INITIAL DATA

Автоматизация технологических и бизнес-процессов
. The canonical task of regression analysis consists in studying the problem of estimation and mathematical description of the influence caused by one or more independent variables (predictors, explanatory variables) on the dependent (explained) variable.
L. G. Raskin, L. V. Sukhomlyn, D. D. Sokolov, V. V. Vlasenko   +3 more
doaj   +1 more source

Interactive Fuzzy Multi Criteria Decision Making Approach for Supplier Selection and Order Allocation in a Resilient Supply Chain

Mathematics, 2019
Modern supply chains are vulnerable to high impact, low probability disruption risks. A supply chain usually operates in such a network of entities where the resilience of one supplier is critical to overall supply chain resilience.
Sonia Irshad Mari, Muhammad Saad Memon, Muhammad Babar Ramzan, Sheheryar Mohsin Qureshi, Muhammad Waqas Iqbal   +4 more
doaj   +1 more source

Approximating a similarity matrix by a latent class model: A reappraisal of additive fuzzy clustering [PDF]

, 2009
Let Q be a given n×n square symmetric matrix of nonnegative elements between 0 and 1, similarities. Fuzzy clustering results in fuzzy assignment of individuals to K clusters.
Braak, C.J.F., ter, Kourmpetis, Y.I.A., Kiers, H.A.L., Bink, M.C.A.M.   +3 more
core   +1 more source

Observer‐Based Adaptive Event‐Triggered Tracking Control for Fuzzy TS Systems With Premise Mismatch

International Journal of Adaptive Control and Signal Processing, EarlyView.
This paper presents an adaptive logistic event‐triggered observer‐based tracking controller for Takagi‐Sugeno fuzzy systems under constrained inputs and network delays. Leveraging a hybrid LMI and Secretary Bird Optimization approach, this strategy significantly minimizes communication overhead and computational burden while ensuring optimal reference ...
Oussama Djadane, Salama Makni, Mourad Kchaou, Ahmed El Hajjaji   +3 more
wiley   +1 more source

Configuring a Circular Economy-based Reverse Logistics Network for the Agri-food Industry based on the Agility and Resilience Dimensions [PDF]

International Journal of Industrial Engineering and Production Research
This study develops a comprehensive circular economy–based reverse logistics network for apple by explicitly integrating agility and resilience considerations under mixed uncertainty.
Samaneh Valipour Khonakdari, Hadi Nasseri, Zohreh Akbari   +2 more
doaj  

A Lightweight Procedural Layer for Hybrid Experimental–Computational Workflows in Materials Science

Advanced Engineering Materials, EarlyView.
We unveil a prototype hybrid‐workflow framework that fuses automatedcomputation with hands‐on experiments. Built atop pyiron, a lightweight, parameterized layer translates procedure descriptions into executable manual steps, syncing instrument settings, human interventions, and data capture in real‐time today.
Steffen Brinckmann, Sarath Menon, Raphael Röske, Johannes Steinhülb, Michael Selzer, Jan Janssen, Tilmann Hickel, Ruth Schwaiger, Jörg Neugebauer   +8 more
wiley   +1 more source

Fuzzy Reliability Optimal Design of Multi-stage Planetary Gear Train with Multi-objective

Jixie chuandong, 2015
The problem of randomness and fuzziness of mechanical design could effectively dealed with through the fuzzy reliability optimization design which established by the combination of fuzzy mathematics method and mechanical optimization technique.Taking the
Hao Liping
doaj