Bioinformatics-Based Screening Approach for the Identification and Characterization of Lipolytic Enzymes from the Marine Diatom Phaeodactylum tricornutum [PDF]
Marine Drugs, 2023 Oleaginous diatoms accumulate lipids of biotechnological interest when exposed to nutrient stress conditions such as nitrogen starvation. While accumulation mechanisms are well-known and have been engineered to improve lipid production, degradation ...
Victor Murison +4 more
doaj +2 more sources
Protective Effects of Neutral Lipids from Phaeodactylum tricornutum on Palmitate-Induced Lipid Accumulation in HepG2 Cells: An In Vitro Model of Non-Alcoholic Fatty Liver Disease [PDF]
MoleculesNon-alcoholic fatty liver disease (NAFLD), often associated with obesity, has become a serious public health matter. NAFLD is characterized by an excessive lipid accumulation in hepatocytes, mainly stored as triglycerides.
Marion Peyras +6 more
doaj +2 more sources
TRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of coronavirus disease 2019 [PDF]
New Microbes and New Infections, 2021 Diagnostic testing is important for managing the 2019 novel coronavirus (SARS-CoV-2). We developed an optimized protocol for SARS-CoV-2 RNA extraction from the surface of the respiratory mucosa with nasopharyngeal swabs and compared the sensitivity of ...
A. Amirouche +6 more
doaj +2 more sources
Structural Characterization and Rheological and Antioxidant Properties of Novel Polysaccharide from Calcareous Red Seaweed [PDF]
Marine Drugs, 2022 A novel sulfated xylogalactan (JASX) was extracted and purified from the rhodophyceae Jania adhaerens. JASX was characterized by chromatography (GC/MS-EI and SEC/MALLS) and spectroscopy (ATR-FTIR and 1H/13C NMR) techniques.
Faiez Hentati +10 more
doaj +2 more sources
Plant-Derived Hydrolysates Are a Suitable Replacement for Tryptone N1 in Recombinant Protein Expression Using Human Embryonic Kidney (HEK293-6E) Cells [PDF]
BioTechHuman embryonic kidney (HEK293) cells are a widespread choice for recombinant protein expression. To optimise yields, the hydrolysate Tryptone N1 (TN1) is commonly added post-transfection. TN1 is obtained by controlled enzymatic digestion of casein.
Shafqat Shabir +4 more
doaj +2 more sources
Natural deep eutectic solvents as a green extraction of polyphenols from spent coffee ground with enhanced bioactivities [PDF]
Frontiers in Plant Science, 2023 Conventional extraction techniques are usually based on highly pollutant and/or flammable organic solvents. Therefore, alternative environmentally friendly extraction methods are of particular interest for the recovery of bioactive compounds for their ...
Aitor García-Roldán +3 more
doaj +2 more sources
Morpho-Anatomical Modulation of Seminal Roots in Response to Water Deficit in Durum Wheat (Triticum turgidum var. durum) [PDF]
PlantsThe productivity of durum wheat in Mediterranean regions is greatly reduced by water deficits that vary in intensity and time of occurrence. The development of more tolerant cultivars is the main solution for fighting these stresses, but this requires ...
Oum Cheikh Felouah +6 more
doaj +2 more sources
Short-term impacts of embryonic thermal manipulation in mule duck, a kinetic study: new tools for metabolic programming [PDF]
BMC GenomicsBackground Temperature changes during embryogenesis, through a process called embryonic thermal programming, can modify the long-term thermotolerance of broilers or the hepatic metabolism of mule ducks.
C. Andrieux +5 more
doaj +2 more sources
Nutritional Composition, Physicochemical Properties, Antioxidant Activity, and Sensory Quality of Matricaria chamomilla-Enriched Wheat Bread [PDF]
FoodsThis study evaluates the effects of fortifying bread with different concentrations (3%, 10%, and 30%) of Matricaria chamomilla L. (MC) infusion and powder, derived from the plant’s aerial parts (stem, leaf, and flower). UPLC/MS-MS analysis of MC infusion
Khawla Kerbab +6 more
doaj +2 more sources
BD GeniVeg : une base de données française sur les ouvrages de protection de berges en génie végétal [PDF]
Sciences, Eaux & Territoires, 2021 Depuis 2017, INRAE et l'Office français de la biodiversité développent une base de données recensant les ouvrages français de génie végétal pour la protection des berges de cours d'eau : BD GeniVeg.
D. JAYMOND +6 more
doaj +2 more sources